本文選題：哮喘　切入點：疾病易感性　出處：《中國全科醫(yī)學》2016年36期

【摘要】：背景有研究證實,白介素6受體(IL-6R)基因中的rs4129267位點多態(tài)性與哮喘易患性顯著相關,但尚無評估rs4129267位點多態(tài)性在漢族兒童中分布情況及其與血清IgE水平相關性的研究。目的研究IL-6R基因rs4129267位點多態(tài)性與漢族兒童哮喘易患性及血清IgE水平的關系。方法選取2013—2014年于廣東省婦幼保健院兒科就診的哮喘患兒410例為哮喘組,同期選取廣東省婦幼保健院住院的非哮喘患兒513例為對照組。提取兩組患兒基因組DNA,采用SNa Pshot法對IL-6R基因rs4129267、rs527236128位點進行基因分型,采用酶聯(lián)免疫吸附試驗(ELISA)法檢測兩組患兒血清總IgE水平。結果兩組患兒rs4129267位點和rs527236128位點的基因型分布均符合Hardy-Weinberg平衡(χ~2=3.264、2.135,P0.05)。兩組患兒rs4129267位點基因型頻率和等位基因頻率比較,差異有統(tǒng)計學意義(P0.05)。兩組患兒rs527236128位點基因型頻率和等位基因頻率比較,差異無統(tǒng)計學意義(P0.05)。Logistic回歸分析結果顯示,TT基因型是兒童哮喘的影響因素(P0.05)。對照組患兒血清總IgE水平低于哮喘組(P0.05)。對照組、哮喘組組內不同基因型患兒血清總IgE水平比較,差異無統(tǒng)計學意義(P0.05);對照組各基因型患兒血清總IgE水平均低于哮喘組(P0.05)。結論 IL-6R基因rs4129267位點多態(tài)性與漢族兒童哮喘易患性有關,而與哮喘患兒血清IgE水平無關。
[Abstract]:Background rs4129267 polymorphism in the interleukin-6 receptor IL-6R) gene has been confirmed to be significantly associated with asthma susceptibility. However, the distribution of rs4129267 locus polymorphism in Han children and its correlation with serum IgE level have not been evaluated. Objective to study the relationship between IL-6R gene rs4129267 locus polymorphism and asthma susceptibility and serum IgE level in Han children. Methods 410 asthmatic children in Guangdong Maternal and Child Health Hospital from 2013 to 2014 were selected as asthma group. In the same period, 513 non-asthmatic children in Guangdong Maternal and Child Health Care Hospital were selected as control group. Genomic DNA was extracted from two groups of children, and IL-6R gene rs4129267rs527236128 was genotyped by SNa Pshot method. Serum total IgE levels were detected by Elisa. Results the genotype distribution of rs4129267 and rs527236128 loci in the two groups were in accordance with the Hardy-Weinberg equilibrium (蠂 2 / 264 / 2.135 P 0.05). The frequencies of rs4129267 loci and alleles were compared between the two groups. The frequency of genotype and allele of rs527236128 locus were compared between the two groups. The results of logistic regression analysis showed that the serum total IgE level in the control group was lower than that in the asthmatic group. The serum total IgE level in the asthmatic group was lower than that in the control group, and the serum total IgE level in the asthmatic group was higher than that in the control group. The serum total IgE level of the control group was lower than that of the asthmatic group (P 0.05). Conclusion the polymorphism of rs4129267 locus of IL-6R gene is related to the susceptibility to asthma in Han nationality children, but not to the serum IgE level of asthmatic children.
【作者單位】： 廣東省婦幼保健院廣東省兒童醫(yī)院廣東省婦產(chǎn)醫(yī)院兒科;
【基金】：廣東省自然科學基金資助項目(s2013010012670)
【分類號】：R725.6
，

本文編號：1661405