本文選題：矢竹　切入點：轉(zhuǎn)錄組　出處：《南京林業(yè)大學學報(自然科學版)》2017年05期 　論文類型：期刊論文

【摘要】：【目的】揭示竹子地下莖生長發(fā)育的分子機制。【方法】利用高通量轉(zhuǎn)錄組測序?qū)κ钢窈煌l(fā)育階段節(jié)的地下莖轉(zhuǎn)錄組圖譜進行了分析研究,并利用qRT-PCR對節(jié)間生長相關(guān)基因進行表達模式分析。【結(jié)果】共獲得了11 976條平均長度為1 008 bp的單基因簇(unigenes)。NCBI注釋顯示,63.8%的單基因簇具有注釋結(jié)果,其中16 254條unigenes被注釋到137個KEGG(京都基因與基因組百科全書)代謝通路中。Map Man分析共獲得了1 864個轉(zhuǎn)錄因子及603個激素代謝及信號轉(zhuǎn)導相關(guān)單基因簇。在功能基因方面,共獲得564個與細胞壁構(gòu)建相關(guān)的代謝基因,其中92個與木質(zhì)素合成相關(guān)。此外,對9個與赤霉素、細胞壁合成及細胞骨架組織相關(guān)的基因,通過實時熒光定量PCR,分析了它們在節(jié)間不同發(fā)育階段的表達模式。結(jié)果顯示:細胞壁、細胞骨架下游功能相關(guān)基因在矢竹地下莖節(jié)間快速生長階段表達最高;而赤霉素合成及信號傳導相關(guān)基因則在節(jié)間伸長起始階段最高�！窘Y(jié)論】矢竹地下莖生長發(fā)育受到從各類激素、轉(zhuǎn)錄因子到其下游功能基因等組成的復雜分子網(wǎng)絡(luò)的協(xié)同調(diào)控。
[Abstract]:[objective] to reveal the molecular mechanism of the growth and development of underground stems of bamboo. [methods] High-throughput transcriptome sequencing was used to analyze the transcriptional maps of underground stems with different development stages. The expression patterns of internode growth-related genes were analyzed by qRT-PCR. [results] A total of 11,976 single gene clusters with an average length of 1 008bp were obtained, indicating that 63.8% of the single gene clusters had annotated results. Of these, 16,254 unigenes were annotated into 137 KEGG (Kyoto Gene and Genome Encyclopedia) metabolic pathways. Map Man analysis obtained 1 864 transcription factors and 603 single gene clusters related to hormone metabolism and signal transduction. A total of 564 metabolic genes related to cell wall construction were obtained, of which 92 were related to lignin synthesis. In addition, 9 genes related to gibberellin, cell wall synthesis and cytoskeleton tissue were identified. The expression patterns of PCRs in different stages of internode development were analyzed by real-time fluorescence quantitative PCR.The results showed that the expression of genes related to the downstream function of cytoskeleton was the highest in the rapid growth stage of Internode of sagittal bamboo. The genes related to gibberellin synthesis and signal transduction were the highest at the beginning of Internode elongation. [conclusion] the growth and development of rhizome of sagittal bamboo is controlled by a complex molecular network composed of hormones, transcription factors and its downstream functional genes.
【作者單位】： 南方現(xiàn)代林業(yè)協(xié)同創(chuàng)新中心南京林業(yè)大學竹類研究所;
【基金】：國家林業(yè)公益性行業(yè)科研專項項目(201504106) 國家自然科學基金項目(31301808,31670602) 江蘇高校優(yōu)勢學科建設(shè)工程資助項目(PAPD)
【分類號】：S795
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本文編號：1652089