本文選題：伊犁馬　切入點(diǎn)：胃蠅屬幼蟲　出處：《中國(guó)獸醫(yī)學(xué)報(bào)》2017年08期 　論文類型：期刊論文

【摘要】：為了解伊犁馬體內(nèi)寄生胃蠅屬幼蟲的種類及馬胃蠅蛆的分類。本試驗(yàn)通過(guò)對(duì)鑒定出的優(yōu)勢(shì)種馬胃蠅三期幼蟲進(jìn)行流行病學(xué)及COI基因分析,采用鄰接法(NJ)構(gòu)建系統(tǒng)發(fā)育樹(shù)。結(jié)果顯示,共收集16 755個(gè)三期幼蟲,總感染率為100%,共發(fā)現(xiàn)4種胃蠅屬幼蟲,混合感染現(xiàn)象嚴(yán)重。鼻胃蠅幼蟲為優(yōu)勢(shì)種,優(yōu)勢(shì)度為53.17%,寄生部位更傾向于賁門處,其他3種則無(wú)規(guī)律地寄生于幽門處。測(cè)定的鼻胃蠅蛆、腸胃蠅蛆、獸胃蠅蛆、紅尾胃蠅蛆的COI基因序列與GenBank上的序列(登錄號(hào):GU265752.1、KR230402.1、KU578262.1、KT946620.1)的核苷酸同源性分別為99%,99%,99%,100%;系統(tǒng)進(jìn)化分析結(jié)果表明,本試驗(yàn)測(cè)定與GenBank上發(fā)表的胃蠅屬的相應(yīng)蠅蛆種聚類;即腸胃蠅和紅尾胃蠅首先聚在一起,再與鼻胃蠅聚在一起,最后三者再和獸胃蠅聚在一起。當(dāng)以COI序列為靶標(biāo)時(shí),內(nèi)群外群分別形成獨(dú)立的進(jìn)化分支。該試驗(yàn)結(jié)果可為胃蠅屬的分類提供參數(shù)。
[Abstract]:In order to understand the species of the parasitic larvae of the genus Gastromyza in Yili horse and the classification of the larvae of the genus Musca, this experiment was carried out by epidemiological analysis and COI gene analysis of the third stage larvae of the dominant stallion. Phylogenetic tree was constructed by using NJ). The results showed that 16,755 third stage larvae were collected and the total infection rate was 100. Four species of the genus Gastrozoa larvae were found, and the mixed infection was serious. The larva of the nasal stomach fly was the dominant species. The dominant degree was 53.17, the parasitic site was more inclined to the cardia, the other three species were irregular parasitic in the pylorus. The nasal and stomach maggots, the enterogastric fly maggots, and the animal stomach fly maggots were measured. The nucleotide homology of the COI gene sequence of the maggots in the red tail and the sequence on the GenBank (accession number: GU265752.1 + KR230402.1 + KU578262.1 + KT946620.1) was 999999946620.1.The results of phylogenetic analysis showed that the results of phylogenetic analysis showed that this experiment was similar to the corresponding species of Musca published on GenBank. That is, the stomach fly and the red tail fly first get together, then the nose stomach fly, and the last three get together with the animal stomach fly. When the COI sequence is used as the target, The results of this experiment can provide parameters for the classification of the genus Gypogaster.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)學(xué)院;新疆昭蘇縣畜牧局;
【基金】：國(guó)家自然科學(xué)基金-新疆聯(lián)合基金重點(diǎn)資助項(xiàng)目(U1403283,U1503188)
【分類號(hào)】：S858.21

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本文編號(hào)：1643153