本文選題：四環(huán)素抗性基因　切入點(diǎn)：芬頓氧化　出處：《中國(guó)環(huán)境科學(xué)》2017年09期 　論文類型：期刊論文

【摘要】：以四環(huán)素抗性基因tet(L)、tet(E)為研究對(duì)象,考察了芬頓氧化技術(shù)對(duì)四環(huán)素抗性基因廢水深度處理的效果.采用定量PCR與DGGE技術(shù)檢測(cè)了芬頓法處理前后生物處理排水中的四環(huán)素抗性基因的豐度與廢水中菌群豐度的變化.結(jié)果顯示,在最佳反應(yīng)條件:反應(yīng)時(shí)間10min,p H值為5,[H_2O_2]/[Fe~(2+)](物質(zhì)量的比)=8且H2O2的濃度達(dá)到0.2mol/L時(shí),16Sr RNA及四環(huán)素抗性基因(tet genes)的去除效果最好.同時(shí)分析芬頓氧化前后的DGGE圖譜可知,細(xì)菌菌群間的豐度有了明顯的變化.相關(guān)性分析可知,各目標(biāo)基因與單因素間、各目標(biāo)基因之間,各樣品中目標(biāo)基因含量的變化與DGGE條帶變化之間都沒有明顯相關(guān)性.結(jié)果表明,芬頓試劑可能不僅僅是通過削減單位水體中抗性菌的濃度來降低抗性基因濃度,還通過其他的方式來去除單位水體中的抗性基因的濃度,這還有待進(jìn)一步研究證明.
[Abstract]:The tetracycline resistance gene (Tet) was studied. The effect of Fenton oxidation on the advanced treatment of tetracycline resistance gene wastewater was investigated. Quantitative PCR and DGGE techniques were used to detect the abundance of tetracycline resistance gene and the flora in wastewater before and after treatment by Fenton method. Changes in abundance. The results show that. Under the optimum reaction conditions: the reaction time is 10 min, the pH value is 5, [H _ S _ 2O _ 2] / [Fe~(2] (the mass ratio is 8 and the concentration of H _ 2O _ 2 is 0.2mol / L), the removal efficiency is best when the concentration of H _ 2O _ 2 is 0.2mol / L. the DGGE profiles before and after Fenton oxidation are also analyzed. The abundance of bacterial flora has changed obviously. The correlation analysis shows that the target genes are among the single factor, the target genes and the target genes. There was no significant correlation between the change of target gene content and the change of DGGE band in each sample. The results showed that Fenton reagent might not only reduce the concentration of resistant bacteria in unit water, but also decrease the concentration of resistant gene. There are other ways to remove the concentration of resistance gene in unit water, which needs further study.
【作者單位】： 中國(guó)環(huán)境科學(xué)研究院城市水環(huán)境科技創(chuàng)新基地;中國(guó)地質(zhì)大學(xué)(北京)水資源與環(huán)境學(xué)院;中國(guó)礦業(yè)大學(xué)(北京)化學(xué)與環(huán)境工程學(xué)院;阿爾伯特大學(xué)土木與環(huán)境工程系;
【基金】：中央級(jí)公益性科研院所基本科研業(yè)務(wù)專項(xiàng)(2016YSKY-027) 國(guó)家環(huán)境保護(hù)環(huán)境微生物利用與安全控制重點(diǎn)實(shí)驗(yàn)室開放基金資助(MARC 2012D008)
【分類號(hào)】：X703

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本文編號(hào)：1630648