本文選題：拉莫三嗪　切入點：皮膚不良反應(yīng)　出處：《寧夏醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的研究拉莫三嗪(LTG)激活T細(xì)胞介導(dǎo)免疫應(yīng)答而誘發(fā)皮膚不良反應(yīng)(cADRs)的可能致病機制。分析在LTG-cADRs患者與LTG耐受者外周血單個核細(xì)胞(PBMCs)中,TCRBV基因表達(dá)的差異。探討LTG-cADRs與TCRBV基因表達(dá)的相關(guān)性。資料與方法收集10例cADRs已治愈達(dá)6周以上的LTG-cADRs患者作為病例組,依據(jù)病例對照研究的方法按1:1匹配納入10例LTG耐受者作為對照組,分離病例組與對照組患者PBMCs,給予LTG體外刺激并培養(yǎng),采用ELISA法測定病例組與對照組患者PBMCs分泌IFN-γ、IL-5及TNF-β的情況,運用實時熒光定量逆轉(zhuǎn)錄PCR技術(shù)(RT-qPCR)的SYBR GreenⅠ熒光染料法檢測TCRBV基因的表達(dá)。用兩獨立樣本t(或t')檢驗比較兩組IFN-γ、IL-5及TNF-β的濃度差異。采用2-ΔΔCt相對定量法計算在病例組與對照組中,TCRBV基因的相對表達(dá)量。用Mann-Whitney U檢驗分析兩組TCRBV基因相對表達(dá)量的差異。結(jié)果1、以終濃度為25μg/ml的LTG分別刺激病例組與對照組的PBMCs,發(fā)現(xiàn)病例組細(xì)胞上清液中IFN-γ濃度(1.06±0.09ng/ml)明顯高于對照組(0.86±0.16ng/ml),差異有統(tǒng)計學(xué)意義(P0.05)。病例組細(xì)胞上清液中IL-5和TNF-β的濃度(0.73±0.06ng/ml,0.69±0.05ng/ml)均略高于對照組(0.70±0.07ng/ml,0.65±0.05ng/ml),差異無統(tǒng)計學(xué)意義(P0.05)。2、病例組與對照組TCRBV基因表達(dá)存在差異,TCRBV11、TCRBV15、TCRBV17、TCRBV18、TCRBV19在病例組中的相對表達(dá)量均較對照組高(P0.05)。結(jié)論1、LTG可在體外激活LTG-cADRs患者的PBMCs分泌IFN-γ,提示T細(xì)胞介導(dǎo)的免疫反應(yīng)可能參與了LTG-cADRs的致病機制。2、LTG-cADRs可能與TCRBV11、TCRBV15、TCRBV17、TCRBV18、TCRBV19基因的表達(dá)相關(guān)。
[Abstract]:Objective to study the possible pathogenesis of LTG activation of T cell mediated immune response and induce skin adverse reaction (ADRs), and to analyze the difference of TCR BV gene expression in peripheral blood mononuclear cells (PBMCs) of patients with LTG-cADRs and LTG tolerant patients, and to explore the possible mechanism of TCR BV gene expression in peripheral blood mononuclear cells (PBMCs) of patients with LTG-cADRs and LTG tolerant patients. Data and methods Ten patients with LTG-cADRs who had been cured of cADRs for more than 6 weeks were collected as the case group. According to the method of case-control study, 10 patients with LTG tolerance were selected as control group according to 1: 1 match. PBMCs were stimulated and cultured with LTG in vitro. The secretion of IFN- 緯 IL-5 and TNF- 尾 by PBMCs in case group and control group was measured by ELISA method. The expression of TCRBV gene was detected by real-time fluorescence quantitative reverse transcriptase PCR (RT-qPCR) SYBR Green 鈪，

本文編號：1623244