發(fā)布時(shí)間：2018-03-15 00:32

  本文選題：18β-甘草次酸　切入點(diǎn)：HepG2肝癌細(xì)胞　出處：《北方民族大學(xué)》2016年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：以往研究表明,18β-甘草次酸(18β-Glycyrrhetinic acid,18β-GA)有一定的防癌和抗癌作用,但對(duì)其分子機(jī)制的研究報(bào)道甚少。本課題組前期研究顯示,18β-GA能夠顯著抑制HepG2肝癌細(xì)胞的增殖,并可調(diào)節(jié)一些癌癥相關(guān)基因的表達(dá)。在此基礎(chǔ)上,本論文研究了18β-GA對(duì)HepG2肝癌細(xì)胞凋亡及其對(duì)細(xì)胞凋亡相關(guān)基因c-jun表達(dá)的影響,初步探討了18β-GA對(duì)HepG2肝癌細(xì)胞凋亡的影響及其分子機(jī)制。本論文主要研究結(jié)果如下:1、18β-GA對(duì)HepG2肝癌細(xì)胞的凋亡誘導(dǎo)作用。分別以60μg/mL的18β-GA處理HepG2肝癌細(xì)胞0 h、6 h、12 h、24 h、48 h(0 h組為對(duì)照組),之后采用流式細(xì)胞術(shù),檢測(cè)18β-GA對(duì)HepG2肝癌細(xì)胞的凋亡誘導(dǎo)作用。結(jié)果顯示,與對(duì)照組相比,隨著作用時(shí)間的延長(zhǎng),凋亡誘導(dǎo)率逐漸升高到10.09%、31.79%、42.2%和66.91%,且具有顯著性差異(P㩳0.05)。該結(jié)果表明,18β-GA對(duì)HepG2肝癌細(xì)胞有時(shí)間依賴(lài)性的凋亡誘導(dǎo)作用。2、18β-GA對(duì)Hep G2肝癌細(xì)胞中c-jun基因表達(dá)的影響。分別以60μg/m L的18β-GA處理HepG2肝癌細(xì)胞0 h、6 h、12 h、24 h、48 h(0 h組為對(duì)照組)。采用western blotting和real time-PCR,分別檢測(cè)了c-jun基因的mRNA和蛋白表達(dá)變化。結(jié)果顯示,與對(duì)照組相比,18β-GA處理6 h,12 h,24 h,48 h后,cjun mRNA表達(dá)量分別上調(diào)了2.5倍(P㩳0.05)、2.7倍(P㩳0.05)、3.2倍(P㩳0.05)和6.2倍(P㩳0.05);c-jun蛋白表達(dá)量別上分調(diào)了3.8倍、6.2倍、8.4倍(P㩳0.05)和17.0倍(P㩳0.05)。該結(jié)果表明18β-GA處理能顯著上調(diào)HepG2肝癌細(xì)胞中c-jun的基因表達(dá),并且趨勢(shì)與細(xì)胞凋亡趨勢(shì)呈正相關(guān),提示18β-GA對(duì)HepG2細(xì)胞凋亡的誘導(dǎo)作用可能與其上調(diào)c-jun基因的表達(dá)有關(guān)。
[Abstract]:Previous studies have shown that 18 尾 -Glycyrrhetinic acidinic 18 尾 -GA) has a certain anti-cancer and anticancer effect, but there are few reports on its molecular mechanism. Our previous studies have shown that Zeng18 尾 -GA can significantly inhibit the proliferation of HepG2 hepatoma cells. On the basis of this, the effect of 18 尾 -GA on apoptosis and c-jun expression of HepG2 hepatoma cells was studied. The effect of 18 尾 -GA on apoptosis of HepG2 hepatoma cells and its molecular mechanism were preliminarily studied. The main results of this study were as follows: 1: 1G 尾 -GA induced apoptosis in HepG2 hepatoma cells. HepG2 hepatoma cells were treated with 18 尾 -GA at 60 渭 g / mL for 0 h, 6 h, 12 h, 24 h and 48 h, respectively. The control group was treated with flow cytometry. The apoptosis-inducing effect of 18 尾 -GA on HepG2 hepatoma cells was detected. The results showed that the apoptosis-inducing rate increased gradually to 42.2% and 66.91% with the prolongation of treatment time, and there was significant difference between the two groups. The results showed that the apoptosis of HepG2 hepatoma cells was induced in a time-dependent manner. (2) 18 尾 -GA had an effect on the expression of c-jun gene in HepG2 hepatoma cells. HepG2 hepatoma cells were treated with 60 渭 g / mL 18 尾 -GA for 0 h, 6 h, 12 h, 24 h and 48 h 0 h, respectively. Western blotting and real time-PCR were used to detect the changes of mRNA and protein expression of c-jun gene. Compared with the control group, the expression of cjun mRNA was up-regulated by 2.5-fold, respectively, after treatment with 18 尾 -GA for 6 h, 12 h, 24 h and 48 h, respectively. P0. 05 and 2. 7 times P? P0. 05 and 3. 2 times P? 0. 05) and 6. 2 times P? The expression of c-jun protein was increased by 3.8 times, 6.2 times and 8.4 times, respectively. 0. 05) and 17. 0 times P? The results showed that 18 尾 -GA could significantly up-regulate the expression of c-jun gene in HepG2 hepatoma cells, and the trend was positively correlated with the trend of apoptosis, suggesting that 18 尾 -GA could induce apoptosis of HepG2 cells by up-regulating c-jun gene expression.
【學(xué)位授予單位】：北方民族大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R735.7
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本文編號(hào)：1613662