本文選題：牦牛　切入點：DGAT基因　出處：《農(nóng)業(yè)生物技術學報》2017年05期 　論文類型：期刊論文

【摘要】：乳脂是乳的重要組成部分,高乳脂率是牦牛(Bos grunniens)乳的主要特征。二�；视王；D移酶2(diacylglycerol acyltransferase 2,DGAT2)調控乳中甘油三酯(triacylglyceride,TRIG)合成,是乳脂肪性狀的重要候選基因。本實驗應用聚合酶鏈式反應-單鏈構象多態(tài)性(polymerase chain reaction-single strand conformation polymorphism,PCR-SSCP)技術檢測甘南牦牛、天祝白牦牛及大通牦牛DGAT2基因多態(tài)性,并分析基因突變對甘南牦牛乳品質性狀影響。結果表明,三類群牦牛在DGAT2基因intron5區(qū)檢測到A_1、B_1、C_1 3種等位基因,存在c.613-214 AG和c.613-337 CA的SNPs;intron6區(qū)檢測到A_2、B_2、C_2 3種等位基因,存在c.787+616 GA和c.787+691 TG的SNPs;三類群牦牛DGAT2基因兩區(qū)域均表現(xiàn)為中度多態(tài),4個突變位點在群體中形成7種單倍型,各位點間存在連鎖關系且接近于連鎖平衡。甘南牦牛DGAT2基因intron5區(qū)突變影響乳脂率,第2胎攜帶等位基因B_1的個體乳脂率顯著高于未攜帶個體(P0.05);intron6區(qū)突變影響乳脂率、乳蛋白率及總固體物質含量,攜帶等位基因A_2的個體乳脂率、總固體物質含量較高且第2胎母牦牛顯著高于未攜帶者(P0.05),即選留攜帶等位基因A的個體有增加后代群體乳脂率及總固體物質含量的趨勢;第2胎母牦�；蛐虯_2C_2個體乳蛋白率最高,且A_2C_2、B_2B_2型個體顯著高于其他基因型(P0.05);單倍型H1H3個體的乳品質性狀也高于其他單倍型。DGAT2基因intron5和intron6區(qū)突變可作為甘南牦牛乳品質性狀潛在的遺傳標記。本研究為牦牛乳品質性狀候選基因分子遺傳研究積累數(shù)據(jù)。
[Abstract]:Milk fat is an important part of milk, and high milk fat rate is the main characteristic of Bos grunniens) milk. Diacylglycerol acyltransferase 2 (DGAT2) regulates the synthesis of triacylglyceride triacylglyceride triIGl in milk. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCPP-PCR) was used to detect DGAT2 gene polymorphism in Gannan yak, Tianzhu white yak and Datong yak. The effect of gene mutation on milk quality of Gannan yak was analyzed. The results showed that three groups of yaks had detected A1B1C 13 alleles in the intron5 region of DGAT2 gene, and there were 23 alleles in A2B2C2CC6 region of c.613-214 AG and c.613-337 CA, respectively, in the SNPs intron 6 region of C. 613-214 AG and c.613-337 CA. SNPs of c. 787,616 GA and c. 787 691 TG existed, and the two regions of DGAT2 gene were moderately polymorphic, and the four mutation loci formed 7 haplotypes in the populations. There was a linkage relationship and close to linkage equilibrium. The mutation of intron5 region of DGAT2 gene in Gannan Yak affected the milk fat rate. The milk fat rate of individuals carrying allele B-1 in the second embryo was significantly higher than that in the non-carrying individuals (P 0.05 intron 6), and the milk fat rate was affected by the mutation of the intron5 region of the DGAT2 gene in Gannan Yak. Milk protein rate and total solid matter content, individual milk fat rate with allelic A _ 2, The content of total solid matter in the second female yak was significantly higher than that in the non-carrier group P0.05, that is, the individuals with allele A had a tendency to increase the milk fat rate and the total solid matter content of the progeny population. The milk protein rate of the second female yak genotype ACTH 2C2 was the highest. The milk quality traits of the population with A2C2B2 were significantly higher than those of other genotypes P0.05. the milk quality traits of haplotype H1 H3 individuals were also higher than those of other haplotypes. DGAT2 gene intron5 and intron6 region mutations could be used as potential genetic markers for milk quality traits of Gannan yaks. This study is a potential genetic marker for milk quality traits of Gannan yaks. Molecular genetic study on candidate genes for Milk quality traits.
【作者單位】： 甘肅農(nóng)業(yè)大學動物科學技術學院/甘肅省草食動物生物技術重點實驗室;甘南州畜牧科學研究所;
【基金】：甘肅省農(nóng)業(yè)生物技術研究與應用開發(fā)項目(No.GNSW-2013-9;No.GNSW-2016-29) 國家自然科學基金項目(No.31160451) 甘肅省杰出青年科學基金項目(No.1210RJDA008)
【分類號】：S823.85
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本文編號：1607610