發(fā)布時(shí)間：2018-03-13 09:43

  本文選題：宮頸癌　切入點(diǎn)：介入治療　出處：《中國(guó)現(xiàn)代藥物應(yīng)用》2017年18期 　論文類型：期刊論文

【摘要】：目的探討宮頸癌介入治療前后敏感相關(guān)基因的表達(dá)譜,分析宮頸癌介入治療分子機(jī)制。方法 3例接受宮頸癌介入治療患者,治療前后對(duì)其組織標(biāo)本進(jìn)行采集,采取基因表達(dá)譜芯片對(duì)差異表達(dá)基因進(jìn)行篩查,運(yùn)用分子功能注釋系統(tǒng)(MAS)軟件對(duì)差異表達(dá)基因自身功能通路進(jìn)行挖掘。結(jié)果通過對(duì)所提取的總RNA電泳圖譜進(jìn)行分析,顯示有28S、5S以及18S條帶,表示所提取RNA具有較高純度,能在芯片實(shí)驗(yàn)中使用。差異基因共篩選出1162個(gè),和介入治療前相比,治療后939個(gè)下調(diào)基因,223個(gè)上調(diào)基因�；蚬δ芊治鲲@示涉及的主要有脫氧核糖核酸酶(DNA)復(fù)制修復(fù)、BRCA1、ATR及BRCA2基因相關(guān)通路、細(xì)胞有絲分裂、糖酵解代謝路徑以及細(xì)胞周期調(diào)控等。結(jié)論表達(dá)譜芯片能迅速高通量的進(jìn)行篩查,且和介入化療栓塞敏感性有關(guān)的差異表達(dá)基因具有一定相關(guān)性。
[Abstract]:Objective to investigate the expression profile of sensitive genes before and after interventional therapy for cervical cancer, and to analyze the molecular mechanism of interventional therapy of cervical cancer. Methods three patients with cervical cancer received interventional therapy and their tissue specimens were collected before and after treatment. The differentially expressed genes were screened by gene expression microarray, and the functional pathways of differentially expressed genes were mined by molecular function annotation system (MAS) software. Results the total RNA electrophoretic patterns were analyzed. There were 28s 5S and 18s bands, indicating that the extracted RNA was of high purity and could be used in microarray experiments. After treatment, there were 939 down-regulated genes and 223 up-regulated genes. Functional analysis of genes showed that the main genes involved were DNA replication and repair of BRCA1, ATR and BRCA2 gene related pathways, and the cells were mitotic. Conclusion expression microarray can be quickly and high-throughput screening, and the differentially expressed genes related to chemoembolization sensitivity of interventional chemotherapy have some correlation.
【作者單位】： 鞍鋼總醫(yī)院介入科;中國(guó)醫(yī)科大學(xué)附屬第一醫(yī)院鞍山醫(yī)院麻醉科;
【分類號(hào)】：R737.33

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