本文選題：微小RNA　切入點：miR-　出處：《山東醫(yī)藥》2017年03期 　論文類型：期刊論文

【摘要】：目的利用生物信息學(xué)方法預(yù)測miR-210的紅細(xì)胞系(以下簡稱紅系)相關(guān)下游靶基因。方法利用NCBI比對分析miR-210成熟序列在各哺乳動物之間的保守性。運(yùn)用基因數(shù)據(jù)庫篩選miR-210的下游靶基因。采用Cytoscape中的BINGO插件對預(yù)測的miR-210下游靶基因進(jìn)行GO分類富集分析。篩選參與紅系增殖、分化、凋亡的miR-210下游靶基因,采用DAVID和KEGG數(shù)據(jù)庫進(jìn)行信號轉(zhuǎn)導(dǎo)通路富集分析后,預(yù)測miR-210的紅系相關(guān)下游靶基因。利用Targetscan在線工具驗證靶基因與miR-210的相互作用關(guān)系。結(jié)果 miR-210成熟序列在多物種間具有高度保守性。篩選出416個與miR-210相關(guān)性較高的預(yù)測靶基因,其分子功能集中在蛋白結(jié)合、離子跨膜運(yùn)輸活動、酶結(jié)合等,參與的生物學(xué)過程集中在細(xì)胞信號轉(zhuǎn)導(dǎo)、突觸傳導(dǎo)等,參與構(gòu)成的細(xì)胞組分主要有突觸小泡、細(xì)胞質(zhì)、細(xì)胞器等。篩選出26個與紅系增殖、分化、凋亡密切相關(guān)的miR-210下游靶基因,參與調(diào)控的信號通路主要集中于癌癥信號通路、PI3K-Akt信號通路等。初步確定Smad3、Mapk10、Stat5a、Ywhag、Ctgf、Kitl6基因與紅系增殖、分化密切相關(guān),其中Smad3、Mapk10、Stat5a、Ywhag、Kitl可能受miR-210的直接調(diào)控,Ctgf可能受miR-210的間接調(diào)控。結(jié)論初步篩選出miR-210的紅系相關(guān)下游靶基因Smad3、Mapk10、Stat5a、Ywhag、Ctgf、Kitl6,這些靶基因可能與紅系的增殖、分化有關(guān)。
[Abstract]:Objective to predict the downstream target genes associated with erythrocyte lineage (red line) of miR-210 by bioinformatics. Methods NCBI alignment was used to analyze the conservatism of miR-210 mature sequences among mammals. The downstream target genes of miR-210 were selected. The predicted downstream target genes of miR-210 were classified and enriched by BINGO plug-in in Cytoscape. The downstream target genes of miR-210, which were differentiated and apoptotic, were analyzed by DAVID and KEGG database after enrichment of signal transduction pathway. Targetscan online tools were used to verify the interaction between target genes and miR-210. Results miR-210 mature sequences were highly conserved among multiple species. 416 predictive target genes with high correlation with miR-210 were screened. Its molecular functions are concentrated in protein binding, ion transmembrane transport, enzyme binding, etc. The biological processes involved in the biological processes are cell signal transduction, synaptic transmission, etc. The cellular components involved in the formation are mainly synaptic vesicles, cytoplasm, etc. Cell organelles and so on. 26 downstream target genes of miR-210 were screened, which were closely related to the proliferation, differentiation and apoptosis of erythrocytes. The signal pathways involved in the regulation were mainly focused on the PI3K-Akt signaling pathway of cancer. Smad3mapk10Stat5aHYwhaga Kitl may be directly regulated by miR-210 and CTGF may be indirectly regulated by miR-210. Conclusion the downstream target gene Smad3mapk10Stat5aCtgfKitl6 of miR-210 may be related to the proliferation and differentiation of red lines.
【作者單位】： 青海大學(xué)醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(81441116) 青海省科技廳(應(yīng)用)基礎(chǔ)研究計劃項目(2012-Z-729) 青海大學(xué)“123高層次人才培養(yǎng)工程”中青年學(xué)術(shù)帶頭人基金;青海大學(xué)醫(yī)學(xué)院中青年科研基金團(tuán)隊項目(2014-KT-1);青海大學(xué)醫(yī)學(xué)院大學(xué)生科技創(chuàng)新基金項目
【分類號】：Q78

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