本文選題：頭頸部鱗癌　切入點：頭頸部正常組織　出處：《貴州醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:從蛋白、基因水平研究時鐘基因Per1、Per2、Per3在頭頸部鱗癌及正常組織中的表達與臨床特征、分期和生存之間的聯(lián)系,望為臨床評估患者療效及預后提供參考依據(jù)。方法:收集頭頸部鱗癌組織標本20例,頭頸部正常組織標本7例,采用實時熒光定量PCR法檢測Per1、Per2、Per3基因的表達水平,通過相對定量法分析Per1、Per2、Per3基因在頭頸部鱗癌組織及頭頸部正常組織中的表達是否有差異,及頭頸部鱗癌組織中Per1、Per2、Per3基因表達水平與早期、晚期患者之間的關系。同時采用免疫組化法檢測60例頭頸部鱗癌標本及20例頭頸部正常組織標本中Per1、Per2、Per3蛋白表達情況,運用染色程度分級與染色細胞百分比相乘結果測定Per1、Per2、Per3蛋白表達水平,分析頭頸部鱗癌組織及頭頸部正常組織中Per1、Per2、Per3蛋白之間的關系,并從蛋白水平分析其與患者臨床特征、腫瘤臨床分期、即刻療效和生存之間的關系。結果:實時熒光定量PCR法檢測顯示Per1、Per2、Per3基因在頭頸部鱗癌組織中及頭頸部正常組織中均有表達,但頭頸部鱗癌組織中Per1、Per2、Per3基因的表達較正常組織明顯下降(P0.01),晚期患者中Per1、Per2、Per3基因下調水平均高于早期患者,且Per2、Per3基因下調程度明顯高于早期患者,差異有統(tǒng)計學意義(P0.01)。運用免疫組化法檢測表明頭頸部鱗癌組織中Per1、Per2、Per3蛋白陽性表達率明顯低于正常組織中的陽性表達率,差異有統(tǒng)計學意義(P0.05),且頭頸部鱗癌組織中Per1、Per2、Per3蛋白陽性表達與腫瘤臨床分期有明顯相關性(P0.05),但其與患者年齡、性別無關。即刻療效方面,Per1、Per2、Per3蛋白陽性組患者其治療有效率高于陰性組患者,其結果具有統(tǒng)計學意義(P0.05)。頭頸部鱗癌患者中Per1、Per2、Per3蛋白陽性組患者的1、2、3年OS明顯高于陰性組,差異有統(tǒng)計學意義(P0.05);Per1、Per2、Per3蛋白陽性組患者的1、2、3年PFS、RFS均明顯高于陰性組,差異有統(tǒng)計學意義(P0.05)。結論:Per家族基因表達的降低可能與頭頸部鱗癌的發(fā)生具有相關性,且在晚期患者中其Per2、Per3基因的下調程度高于早期頭頸部鱗癌患者;Per家族蛋白表達陽性的頭頸部鱗癌患者較陰性患者其可能具有更長的OS及PFS,RFS,而這也提示Per家族基因與頭頸部鱗癌的預后中具有重要作用。
[Abstract]:Objective: to study the relationship between the expression of clock gene Per1, Per2P3 and clinical features, staging and survival in head and neck squamous cell carcinoma and normal tissues at the protein and gene levels. Methods: 20 cases of head and neck squamous cell carcinoma and 7 cases of normal head and neck tissues were collected to detect the expression of Per1 PER2P 3 gene by real-time fluorescence quantitative PCR. The relative quantitative analysis was used to analyze the difference of the expression of Per1mPer2P3 gene in head and neck squamous cell carcinoma and normal head and neck tissues, and the expression level and early stage in head and neck squamous cell carcinoma. Immunohistochemical method was used to detect the expression of Per1 and Per2P3 protein in 60 cases of head and neck squamous cell carcinoma and 20 cases of normal tissues of head and neck. The expression level of Per1 PER2P 3 protein in head and neck squamous cell carcinoma and normal tissue of head and neck were determined by staining grade and percentage of stained cells. The relationship between Per1 and Per2 Per3 protein in head and neck squamous cell carcinoma and normal tissue of head and neck was analyzed, and the clinical features of patients were analyzed from the protein level. Results: real time fluorescence quantitative PCR assay showed that the expression of Per1mPer2p3 gene was detected in head and neck squamous cell carcinoma tissues and normal head and neck tissues. But in head and neck squamous cell carcinoma, the expression of Per1, Per2, P0. 01, P0. 01, and the down-regulation level of Per1, Per2, Per2, Per3, and the down-regulation of Per2, P0. 01, and the down-regulation of Per2, P0. 01, were significantly lower than those in the early stage of squamous cell carcinoma. The difference was statistically significant (P 0.01). The positive expression rate of Per1, Per2, Per3 protein in head and neck squamous cell carcinoma tissues was significantly lower than that in normal tissues, and the positive expression rate in head and neck squamous cell carcinoma tissues was significantly lower than that in normal tissues. The difference was statistically significant (P 0.05), and the positive expression of Per1 / Per2 / Per3 protein in head and neck squamous cell carcinoma tissues was significantly correlated with the clinical stage of the tumor (P 0.05), but it was associated with the age of the patients. The effective rate of treatment was higher in the positive group than that in the negative group, and the results were statistically significant (P 0.05). In the patients with head and neck squamous cell carcinoma, the results were significantly higher than those in the group with positive protein of Per1P 2 and P 2 + 3, and the OS in 3 years was significantly higher than that in the negative group. The difference was statistically significant (P 0.05) in the patients with positive P0. 05% Per1 and Per2 + Per3 protein, and in 3 years, the RFS of PFSn was significantly higher than that of the negative group, and the difference was statistically significant (P 0. 05). Conclusion the decrease of the gene expression of the weight per family may be related to the occurrence of squamous cell carcinoma of the head and neck. Moreover, the down-regulation of Per2P3 gene in advanced patients was higher than that in patients with head and neck squamous cell carcinoma with positive protein expression of per family. It may have longer OS and PFS- FRFSs in patients with head and neck squamous cell carcinoma than in patients with negative expression. This also suggests that Per family genes are more likely to be present in patients with squamous cell carcinoma of head and neck than those in patients with negative expression of per family proteins. It plays an important role in prognosis of head and neck squamous cell carcinoma.
【學位授予單位】：貴州醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.91

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