本文選題：甘草水提物　切入點：小分子RNA　出處：《中國中藥雜志》2017年09期 　論文類型：期刊論文

【摘要】：微小RNA(micro RNA,miRNA)是一類在轉(zhuǎn)錄后水平調(diào)節(jié)基因表達(dá)的非編碼小分子RNA,其調(diào)控基因表達(dá)、影響細(xì)胞活性的功能已為人們所重視。近年來miRNA的跨界調(diào)控成為新的研究方向,為人們更全面地認(rèn)識和了解miRNA的功能提供了新的視角。植物miRNA由于通常經(jīng)過了甲基化修飾,因此較為穩(wěn)定,不易降解。前期研究表明,甘草干燥藥材的水煎劑中存在著豐富的小分子RNA,這為了解甘草的作用機制提供了新的思路。在本研究中,利用從甘草水煎劑中提取的小分子RNA以及合成的miRNA模擬物(mimic)作用于分離自健康人的外周血單核細(xì)胞(peripheral blood mononuclear cell,PBMC),然后利用RT-PCR對其模式識別受體(PRR)基因以及部分轉(zhuǎn)錄因子、信號分子和細(xì)胞因子的基因表達(dá)變化進(jìn)行了分析。結(jié)果表明甘草miRNA對人免疫細(xì)胞的基因表達(dá)具有明顯的調(diào)節(jié)作用,能夠顯著抑制T細(xì)胞分化相關(guān)基因的表達(dá),以及炎癥和凋亡相關(guān)基因的表達(dá)。這為進(jìn)一步更全面地了解甘草的作用機制以及中藥新藥的研制帶來了新的思路。
[Abstract]:Small RNA(micro RNAs are a kind of non-coding small molecule RNAs that regulate gene expression at post-transcriptional level. The function of regulating gene expression and affecting cell activity has been paid attention to. Recently, the cross-border regulation of miRNA has become a new research direction. It provides a new perspective for a more comprehensive understanding and understanding of the functions of miRNA. Plant miRNA is stable and difficult to degrade because it is usually modified by methylation. There are abundant small molecule RNAs in the decoction of licorice dried herbs, which provides a new idea for understanding the mechanism of licorice. RNA, a small molecule extracted from Glycyrrhiza uralensis decoction, and mimicoid, a synthetic miRNA mimicoid, were used to treat peripheral blood monocytes isolated from healthy individuals, and then RT-PCR was used to identify its pattern recognition receptor (PRRR) gene and some transcription factors. The changes of gene expression of signal molecules and cytokines were analyzed. The results showed that Glycyrrhiza uralensis (miRNA) could significantly regulate the gene expression of human immune cells and inhibit the expression of genes related to T cell differentiation. And the expression of inflammatory and apoptosis-related genes, which provides a new idea for the further understanding of the mechanism of licorice and the development of new drugs of traditional Chinese medicine.
【作者單位】： 廣東藥科大學(xué)生命科學(xué)與生物制藥學(xué)院廣東省生物技術(shù)候選藥物研究重點實驗室;
【基金】：國家自然科學(xué)基金項目(31100664,31300737,81303292) 廣東省自然科學(xué)基金項目(10151022401000024,2014A030313586) 廣東省科技計劃項目(2016A020215157)
【分類號】：R285

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 丁淑芹;金榮華;孫煥芹;李寧;;黃芪甘草水提物的抗誘變作用[J];醫(yī)學(xué)理論與實踐;2008年08期

2 丁淑芹;龐慧民;朱玉琢;;黃芪甘草水提物抗誘變作用[J];中國公共衛(wèi)生;2006年09期

3 ;[J];;年期

相關(guān)會議論文 前1條

1 陳衛(wèi)平;奚麗君;;甘草對藥物代謝Ⅰ相反應(yīng)酶系相關(guān)基因表達(dá)的影響[A];第二屆臨床中藥學(xué)學(xué)術(shù)研討會論文集[C];2009年

，

本文編號：1585082