發(fā)布時間：2018-03-08 02:15

  本文選題：綿羊　切入點：ZBED6基因　出處：《石河子大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：為明確綿羊ZBED6基因的遺傳變異,探討ZBED6基因的遺傳變異與綿羊主要胴體性狀的相關(guān)性,本論文選擇了新疆地方品種阿勒泰羊與特克賽爾羊的雜交后代(橫交F2代、回交F2代2個群體)的225個個體為主要研究對象,以綿羊ZBED6基因為候選基因,旨在查明ZBED6基因的分子遺傳特征及其在群體間的差異,分析該基因變異對所選綿羊胴體性狀的影響,評價其作為所選綿羊重要生產(chǎn)性狀分子遺傳標記的利用價值。本研究的主要研究內(nèi)容和結(jié)果如下:1.試驗群體胴體性狀的測定和影響因素分析試驗測定了170只橫交F2代群體和55只回交F2代群體的7個主要的胴體性狀,包括胴體重量、背部脂肪厚度、眼肌面積、尾脂重量、胴體長、背部肌肉厚、背最長肌質(zhì)量。比較了不同雜交方式對于胴體性狀的影響發(fā)現(xiàn)橫交F2群體的眼肌面積顯著大于回交F2代群體(P0.05),橫交F2代背部肌肉厚度極顯著大于回交F2代群體(P0.01),橫交F2代背最長肌重量極顯著大于回交F2代(P0.01),橫交F2代尾脂重量極顯著小于回交F2代群體(P0.01),橫交F2代胴體長比回交F2代群體長約1.65cm,接近于顯著水平(P=0.05),兩個雜交群體中的背部脂肪厚度和胴體重量差異不顯著(P0.05);比較了不同性別對胴體性狀的影響,發(fā)現(xiàn)公羊的眼肌面積顯著大于母羊(P0.05),公羊的背部脂肪厚度極顯著大于母羊(P0.01),公羊尾脂重量較母羊尾脂重2.87kg,接近于顯著水平(P=0.05),母羊的胴體長極顯著長于公羊(P0.01),不同性別的胴體重量,背部肌肉厚度和背最長肌重量差異不顯著(P0.05)。2.綿羊ZBED6基因多態(tài)性檢測針對ZBED6基因的全基因組長為3099bp的基因序列,本研究設(shè)計了5對PCR引物、樣本量為225個個體的DNA進行擴增,經(jīng)對結(jié)果分析后發(fā)現(xiàn)在Z-2引物測序中兩個雜交群體均存在多態(tài)性,在C1723T和A2095G堿基處發(fā)生了堿基突變CT和AG。這兩個突變均為同義突變。經(jīng)卡方檢驗分析,基因型和等位基因分布在2個雜交群體中差異不顯著(P0.05)。由于Z-1、Z-3、Z-4、Z-5兩對引物經(jīng)檢測分析得到的突變頻率較低,該四對引物均不宜進行下一步實驗。3.ZBED6基因多態(tài)性與7個主要胴體性狀的相關(guān)性分析通過對225個個體C1723T和A2095G兩個位點的基因型檢測ZBED6基因多態(tài)性信息,根據(jù)實驗1的結(jié)果,考慮群體和性別影響因素,利用一般線性模型對7個胴體性狀與ZBED6基因多態(tài)性進行關(guān)聯(lián)分析。統(tǒng)計分析表明,這2個位點與7個胴體性狀無關(guān)聯(lián)性。ZBED6基因這2個突變位點是否與其他胴體性狀相關(guān),需要擴大樣本數(shù)量并且與其余胴體表型進行關(guān)聯(lián)分析驗證。本論文結(jié)論:試驗選取新疆地方品種阿勒泰羊與特克賽爾羊雜交后代的225個個體的耳組織樣品進行ZBED6基因SNPs檢測,并與該群體屠宰獲得的7個胴體性狀進行關(guān)聯(lián)分析。研究表明,ZBED6基因編碼區(qū)在受試群體中存在多態(tài)性,在C1723T和A2095G堿基處發(fā)生了堿基突變CT和AG,這兩個位點的基因型與胴體性狀不相關(guān)。同時,本研究對供試綿羊群體間及其性別對胴體性狀的影響的分析顯示,群體間眼肌面積差異顯著,尾脂重、背部肌肉厚、背最長肌重差異極顯著。其中,橫交F2代羊群體在眼肌面積、背最長肌重、背部肌肉厚性狀方面優(yōu)于回交F2代群體,回交F2代群體尾脂重明顯高于橫交代羊群體;不同性別間眼肌面積、胴體長、背部脂肪厚度差異極顯著。公羊眼肌面積優(yōu)于母羊,但其背部脂肪厚度大于母羊。由此可見,橫交F2代羊能夠滿足綿羊產(chǎn)肉相關(guān)性狀的選育。
[Abstract]:For the clear genetic variation of ZBED6 gene in sheep, to investigate the relationship between genetic variation and carcass traits of main sheep ZBED6 gene, this paper chose the hybrid offspring of Xinjiang local varieties of Aletai sheep and Texel Sheep (cross backcross F2 generation, F2 generation 2 groups) and 225 individuals as the main object of study, to sheep ZBED6 gene as a candidate gene, aimed at identifying the molecular genetic features of ZBED6 gene and the difference between groups, analysis of the impact of the genetic variation of selected sheep carcass traits, evaluate the utilization value of the selected sheep as important production traits of molecular genetic markers. The main research contents and results are as follows: the determination of 7 major and the 170 cross carcass traits in F2 generation and 55 backcross F2 populations were measured in 1. test groups analysis of factors affecting carcass traits, including carcass weight, backfat thickness, eye Muscle area, tail fat weight, carcass length, back muscle thickness and longissimus muscle quality. Compare the different ways of hybridization influence on carcass traits that cross F2 groups were significantly greater than the eye muscle area (P0.05) of backcross F2 generation, F2 generation cross back muscle thickness was significantly higher than that of F2 group (back P0.01), cross F2 longissimus muscle weight was significantly higher than that of backcross F2 generation (P0.01), cross F2 tail fat weight was significantly lower than that of backcross F2 generation (P0.01), cross F2 generation backcross F2 generation carcass length ratio is about 1.65cm, close to significant level (P=0.05), two a hybrid group back fat thickness and carcass weight did not differ significantly (P0.05); the influence of different gender on carcass traits were compared, found that the ram of the eye muscle area was significantly greater than ewes (P0.05), the back fat thickness of rams was significantly higher than that of ewes (P0.01), sheep tail fat weight than sheep tail fat weight 2.87kg That is close to significant level (P=0.05), ewe carcass length was significantly longer than the RAM (P0.01), carcass weight of different sex, back muscle thickness and longissimus muscle weight had no significant difference (P0.05) of.2. sheep ZBED6 gene polymorphism detection of whole genome length for ZBED6 gene sequence of 3099bp genes, this research 5 pairs of PCR primers were designed, samples were amplified for 225 individuals of DNA, through the analysis of the results found in the Z-2 two hybrid population sequencing primers were found polymorphic, the mutation of CT and AG. of the two mutations were synonymous mutations in C1723T and A2095G bases. By chi square test analysis of genotype and allele distribution in 2 hybrid groups had no significant difference (P0.05). The Z-1, Z-3, Z-4, Z-5, two pairs of primers were detected by analysis of the mutation frequency is low, the four pairs of primers of.3.ZBED6 gene were not suitable for the next experiment Correlation analysis of normality and the 7 main carcass traits by the genotypes of 225 individuals of C1723T and A2095G two loci of ZBED6 gene polymorphism detection information, according to the results of the 1 experiment groups and gender influence factors, analyzing the correlation between the 7 carcass traits and ZBED6 gene polymorphism by using general linear model statistics. Analysis shows that these 2 loci and 7 carcass traits were unrelated.ZBED6 genes of the 2 mutations are associated with other carcass traits, need to expand the sample number and correlation analysis verification with the rest of the carcass phenotype. Conclusion: the experiment selects Xinjiang local varieties of Aletai sheep and Texel Sheep hybrids 225 individual ear tissue samples of ZBED6 SNPs gene detection, and 7 carcass traits were obtained with the group slaughter for correlation analysis. The results show that the ZBED6 gene encoding region in the tested group The polymorphism of the body, the mutation of CT and AG in C1723T and A2095G at base, not related to genotype and carcass traits of these two sites. At the same time, the research and Analysis on the effects of the tested sheep populations and sex on carcass traits showed significant difference between groups of eye muscle area, fat tail weight, back muscle thickness, the difference of longissimus muscle weight significantly. Among them, cross the F2 generation of sheep populations in the loin eye area, longissimus muscle weight, back muscle thickness traits than backcross progeny F2, backcross F2 generation tail fat weight was significantly higher than that of sheep cross account groups; gender differences and eye muscle area carcass length, back fat thickness, the difference was very significant. The eye muscle area is better than that of ram ewe, but the back fat thickness greater than ewes. Thus, cross the F2 generation of sheep meat producing sheep breeding can meet related traits.

【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S826
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本文編號：1581963