本文關(guān)鍵詞：棉花CBF基因的克隆及其轉(zhuǎn)基因煙草的抗寒性分析，由筆耕文化傳播整理發(fā)布。

292

作 物 學(xué) 報(bào) 第37卷

的煙草受冷處理后, 游離脯氨酸和可溶性糖的含量都比野生型有所提高。這說(shuō)明GbCBF1的表達(dá)能夠提高植物對(duì)于低溫的耐受性。另外, 本研究中NOS:GbCBF1轉(zhuǎn)基因煙草在0℃冷處理前后, 可溶性糖含量均大大高于35S:GbCBF1轉(zhuǎn)基因煙草, 而在0℃處理時(shí)的電解質(zhì)滲漏率與35S:GbCBF1轉(zhuǎn)基因煙草卻沒(méi)有明顯差異, 說(shuō)明在轉(zhuǎn)基因煙草中可溶性糖含量的提高并不是植物耐寒性增強(qiáng)的主要原因, 這與Cholewa等[25]

和Wanner等

[26]

的研究結(jié)果一致。

可溶性糖含量與植物耐寒性之間的關(guān)系, 需要進(jìn)一

步的研究。

4 結(jié)論

從棉花品種中棉12、中棉36和海島棉7124中克隆并鑒定了CBF全長(zhǎng)基因。CBF在棉花中以多拷貝的形式存在。GbCBF1基因的表達(dá)受低溫、ABA、干旱和鹽脅迫等多種逆境信號(hào)的誘導(dǎo)。獲得了轉(zhuǎn)GbCBF1基因的煙草, 從而提高了煙草的耐寒性。

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  本文關(guān)鍵詞：棉花CBF基因的克隆及其轉(zhuǎn)基因煙草的抗寒性分析，由筆耕文化傳播整理發(fā)布。