本文選題：甘藍型油菜　切入點：甘油三磷酸酰基轉(zhuǎn)移酶　出處：《中國油料作物學報》2017年04期 　論文類型：期刊論文

【摘要】：為揭示油菜BnGPAT9基因的功能及其在非生物脅迫中的表達變化,克隆甘藍型油菜BnGPAT9基因(GenBank:KX344511.1)并分析表達模式。發(fā)現(xiàn)該基因全長1 131bp,編碼376個氨基酸殘基。序列比對及系統(tǒng)進化樹分析表明BnGPAT9與其它植物的GPAT9有很大的相似性,在生物進化過程中具有很高的保守性。實時熒光定量PCR分析表明,BnGPAT9基因在甘藍型油菜中的表達具有組織特異性,在開花后1~4周幼嫩的種子中表達較強,而在莖、花、葉、根和角果皮中表達較弱;5~6片真葉期盆栽苗的非生物脅迫表達分析顯示,短期干旱和高溫能夠誘導和促進葉片BnGPAT9基因的表達;低溫、6-BA、ABA和鹽脅迫抑制其表達;水漬對BnGPAT9基因的表達影響呈"Z"字形,12h出現(xiàn)最低值,但24h后又開始回升。
[Abstract]:In order to reveal the function of BnGPAT9 gene and its expression changes in abiotic stress in rapeseed, BnGPAT9 gene of Brassica napus L. was cloned into GenBank: KX344511.1) and its expression pattern was analyzed. The total length of the gene was 1131 BP, encoding 376 amino acid residues. Sequence alignment and phylogenetic tree analysis showed that BnGPAT9 was similar to GPAT9 of other plants. The real-time fluorescence quantitative PCR analysis showed that the expression of BnGPAT9 gene was tissue specific in Brassica napus, and was strongly expressed in young seeds 1 week after anthesis, but in stems, flowers and leaves. Abiotic stress analysis showed that short-term drought and high temperature could induce and promote the expression of BnGPAT9 gene in leaves, but low temperature and salt stress inhibited the expression of BnGPAT9 gene. The effect of water on the expression of BnGPAT9 gene was the lowest in 12 h, but it began to rise again after 24 hours.
【作者單位】： 國家油料改良中心湖南分中心;湖南農(nóng)業(yè)大學作物基因工程湖南省重點實驗室;
【基金】：“十三五”國家重點研發(fā)計劃(2016YFD0101300) 國家973計劃(2015CB150200) 湖南省科技計劃(2014FJ1006-3) 湖南省省長專項(湘財農(nóng)指[2016]114) 湖南省研究生科研創(chuàng)新項目(CX2017B376)
【分類號】：S565.4
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本文編號：1571519