本文選題：胃癌　切入點：EBV　出處：《青島大學》2016年碩士論文　論文類型：學位論文

【摘要】：目的:探討TLR2(rs3804099、rs3804100),TLR4(rs11536889、rs10759932)和TLR9(TLR9-1486、TLR9-2848)基因多態(tài)性與胃癌和EBV相關胃癌(Epstein Barr virus-associated GC,EBVa GC)易感性的關系。方法:選用青島地區(qū)EBV陰性的胃癌(EBVn GC)組織、EBVa GC組織及正常健康人群外周血標本作為研究對象,采用PCR結合RFLP的技術檢測TLR2(rs3804099、rs3804100),TLR4(rs11536889、rs10759932)和TLR9(TLR9-1486、TLR9-2848)基因的多態(tài)性;對部分PCR產物進行測序,DNAStar軟件對序列進行對比分析,驗證PCR-RFLP基因分型結果;對實驗結果進行統(tǒng)計處理,比較分析胃癌患者和健康對照組、EBVa GC組織和EBVn GC組織的基因型和等位基因頻率。結果:(1)TLR2 rs3804099的基因型和等位基因頻率在胃癌組與健康對照組間有顯著性差異(χ2=5.617,p=0.018;χ2=6.467,p=0.011),胃癌組C等位的基因頻率及C等位的基因攜帶者頻率均顯著高于正常健康對照組(χ2=6.467,p=0.011;χ2=4.444,p=0.035),并且與野生TT型相比,CC基因型可明顯增加胃癌的發(fā)病率(OR=3.554,95%CI=1.179-10.715)。(2)TLR2rs3804100位點各基因型的頻率、C等位基因及C等位基因攜帶者頻率在胃癌組和正常健康對照組之間無明顯差異(p0.05)。(3)TLR4(rs10759932)基因的多態(tài)性與青島地區(qū)胃癌的發(fā)病率有明顯相關性,攜帶TC和CC基因型可以減少胃癌的易感性(OR=0.483,95%CI=0.289-0.807),C等位基因可能是胃癌的保護基因,攜帶C等位基因可以顯著降低胃癌的罹患風險。(4)TLR4(rs11536889)基因型、等位基因的頻率、攜帶者的頻率在胃癌組和健康對照組間均無顯著差異(P0.05)。(5)胃癌組TLR9(1486T/C)和TLR9(C2848T)基因的多態(tài)性與對照組比較均無顯著差異(P0.05)。(6)TLR2rs3804099、rs3804100;TLR4rs11536889、rs10759932;TLR9-1486、TLR9-2848基因多態(tài)性在EBVn GC與EBVa GC組間均無顯著差異(P0.05)。結論:(1)TLR2 rs3804099基因的多態(tài)性可能與胃癌的發(fā)病率有關,C等位基因可能為胃癌的高�；�,攜帶C等位基因可能會增加胃癌的罹患率。(2)TLR4 rs10759932基因的多態(tài)性可能與胃癌的發(fā)病率有關,C等位基因可能為胃癌的保護基因,攜帶C等位基因可能會降低胃癌的罹患率。(3)TLR2(rs3804099、rs3804100);TLR4(rs11536889、rs10759932);TLR9-1486和TLR9-2848基因多態(tài)性與EBV的感染沒有協(xié)同作用,與EBVa GC的易感性沒有相關性。
[Abstract]:Objective: to investigate the relationship between the polymorphism of TLR2 rs3804099 rs3804100 (TLR4 rs11536889 rs10759932) and TLR9-1486 (TLR9-2848) gene polymorphism and the susceptibility to Epstein Barr virus-associated GCV-a GC of gastric cancer and EBV associated gastric cancer. PCR combined with RFLP was used to detect the polymorphism of TLR2 rs3804099 / rs3804100) and TLR9rs11536889 (rs10759932) and TLR9 / TLR9-1486 (TLR9-2848) respectively, and some PCR products were sequenced by DNAStar software to verify the results of PCR-RFLP genotyping. The genotypes and allelic frequencies of TLR2 rs3804099 in gastric cancer patients and healthy controls were compared and analyzed. Results there were significant differences in genotype and allele frequencies of TLR2 rs3804099 between gastric cancer patients and healthy controls (蠂 2 5.617 p0. 018, 蠂 2 6.467, p0.011, P 0. 011, P 0. 011, P 0. 011, P 0. 011, P 0. 011, P 0. 011, P 0. 011, P 0. 011). The frequency of C allele and C allele carrier in group C were significantly higher than those in normal control group (蠂 2 / 6.467), 蠂 ~ (2 +) = 4.444 / (P = 0.035), and compared with wild TT genotype, the frequency of CC genotype was significantly higher than that of wild TT genotype. The incidence of gastric cancer was significantly higher than that of control group (P < 0.05), and the frequency of genotype C of each genotype of TLR2rs3804100 was significantly higher than that of wild-type TT genotype (P < 0.05), which was higher than that of control group (蠂 ~ 2 = 6.467715.2TLR2rs3804100). There was no significant difference in the frequency of locus gene and C allele carrier between gastric cancer group and normal control group. The polymorphism of TLR4 rs10759932) gene was significantly correlated with the incidence of gastric cancer in Qingdao area. Carrying TC and CC genotypes can reduce the susceptibility of gastric cancer. The allele C may be a protective gene for gastric cancer. Carrying C allele can significantly reduce the risk of gastric cancer. There was no significant difference in the frequency of TLR9rs3809rs3809rs3809rs3804100TLR4rs11536889rs1075999rs1075999TLR9-1486TLR9-2848 gene polymorphism between the EBVn GC group and the EBVa GC group. There was no significant difference in the polymorphism of TLR9-1486TLR9-2848 gene between the EBVn GC group and the EBVa GC group (P0.05P > 0.05), and there was no significant difference in the polymorphism of TLR9-1486TLR9-2848 gene between the EBVn GC group and the EBVa GC group. The polymorphism of TLR2 rs3804099 gene may be related to the incidence of gastric cancer. The allele C may be a high risk gene for gastric cancer. Carrying C allele may increase the risk of gastric cancer. The polymorphism of TLR4 rs10759932 gene may be related to the incidence of gastric cancer. Carrying the C allele may reduce the attack rate of gastric cancer. TLR2TLR2ORs3804099 rs3804100 TLR4 rs11536889 rs10759932hp polymorphism and TLR9-2848 gene polymorphisms have no synergistic effect on EBV infection, and there is no correlation between TLR9-1486 polymorphism and susceptibility to EBVa GCC.There is no association between TLR9-1486 and TLR9-1486 gene polymorphisms, but no association between TLR9-1486 and TLR9-1486 gene polymorphism.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R735.2

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本文編號：1564698