本文選題：紅斑丹毒絲菌　切入點(diǎn)：NaOH提取抗原　出處：《微生物學(xué)報(bào)》2016年02期 　論文類型：期刊論文

【摘要】：【目的】為深入研究紅斑丹毒絲菌的免疫保護(hù)性抗原及其致病機(jī)制,采用免疫蛋白組學(xué)技術(shù)鑒定紅斑丹毒絲菌的免疫原性蛋白。【方法】通過SDS-PAGE電泳分離紅斑丹毒絲菌C43065株的NaOH提取抗原,用兔抗NaOH提取抗原抗血清經(jīng)Western blot檢測免疫原性蛋白,通過MALDI-TOF質(zhì)譜技術(shù)鑒定蛋白種類,并對部分免疫原性蛋白的編碼基因進(jìn)行克隆和測序�！窘Y(jié)果】通過MALDI-TOF質(zhì)譜技術(shù)從C43065株NaOH提取抗原中鑒定出9個(gè)免疫原性蛋白,分別為Spa A、伴侶蛋白GroEL、烯醇化酶、ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白、丙酮酸脫氫酶復(fù)合物E1、甘油醛-3-磷酸脫氫酶、果糖二磷酸醛縮酶、50S核糖體蛋白L1、30S核糖體蛋白S4。其中烯醇化酶、ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白、甘油醛-3-磷酸脫氫酶和果糖二磷酸醛縮酶已被證實(shí)與鏈球菌、牙齦卟啉單胞菌、腦膜炎奈瑟菌和結(jié)核分枝桿菌的致病性相關(guān)。C43065株伴侶蛋白GroEL、烯醇化酶、ATP結(jié)合盒轉(zhuǎn)運(yùn)蛋白、丙酮酸脫氫酶復(fù)合物E1、甘油醛-3-磷酸脫氫酶和果糖二磷酸醛縮酶編碼基因大小分別為1614、1296、1260、1005和867 bp,與已公布的紅斑丹毒絲菌Fujisawa株相應(yīng)基因的相似度高達(dá)98%�！窘Y(jié)論】本文所鑒定的9個(gè)免疫原性蛋白,為進(jìn)一步開展紅斑丹毒絲菌保護(hù)性抗原及其致病機(jī)制研究奠定基礎(chǔ)。
[Abstract]:[objective] to study the immune protective antigen of erysipelas erythematosus and its pathogenic mechanism. The immunogenicity protein of erysipelas erythematosus was identified by immunoproteomics technique. [methods] the NaOH antigen of Rhizoma erythematosus C43065 strain was isolated by SDS-PAGE electrophoresis, and the immunogenicity protein was detected by Western blot in rabbit anti-#en2# antiserum. The protein types were identified by MALDI-TOF mass spectrometry, and the coding genes of some immunogenic proteins were cloned and sequenced. [results] 9 immunogenic proteins were identified from C43065 strain NaOH by MALDI-TOF mass spectrometry. They are Spa A, chaperone protein Groel, enolase ATPase ATP-binding cassette transporter, pyruvate dehydrogenase complex E1, glyceraldehyde 3-phosphate dehydrogenase. Fructose diphosphate aldolase 50s ribosomal protein L1 / 30s ribosomal protein S4. among them enolase ATP binding cassette transporter, glyceraldehyde 3-phosphate dehydrogenase and fructose diphosphate aldolase have been confirmed to be associated with Streptococcus gingivalis, porphyromonas gingivalis. Pathogenicity of Neisseria meningitidis and Mycobacterium tuberculosis. The coding genes of pyruvate dehydrogenase complex E1, glyceraldehyde 3-phosphate dehydrogenase and fructose diphosphate aldolase were 1614, 1296, 1260,1005 and 867 BP, respectively. The similarity of the genes with the published Fujisawa strain was 98. [conclusion]. Nine immunogenic proteins were identified. It lays a foundation for further research on protective antigen and pathogenic mechanism of erysipelas erythematosus.
【作者單位】： 伊犁師范學(xué)院生物與地理科學(xué)學(xué)院;吉首大學(xué)生物資源與環(huán)境科學(xué)學(xué)院;中國疾病預(yù)防控制中心傳染病預(yù)防控制所;
【基金】：國家自然科學(xué)基金項(xiàng)目(31360613,31072142)~~
【分類號】：S852.6

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