本文關(guān)鍵詞： 水稻 矮稈 基因克隆 轉(zhuǎn)錄因子 AP　出處：《南京農(nóng)業(yè)大學學報》2017年04期 　論文類型：期刊論文

【摘要】：[目的]對水稻矮稈突變體htr進行表型分析與基因克隆,為水稻育種提供新的矮稈基因資源。[方法]對矮稈突變體htr莖稈進行細胞學觀察,并將htr與‘N22’和‘9311’雜交構(gòu)建F2群體,提取隱性極端個體定位基因HTR。[結(jié)果]htr是一個穩(wěn)定遺傳的矮稈突變體,其株高、穗長、千粒質(zhì)量、分蘗數(shù)等性狀均降低,但其葉片變寬,葉色深綠,莖稈增粗。成熟期對htr倒二節(jié)間進行切片觀察發(fā)現(xiàn):相比‘9311’,htr在莖稈的縱軸方向細胞未能正常伸長,橫向細胞數(shù)目增多,細胞變小。遺傳分析表明突變體htr的矮稈性狀受1對隱性單基因控制。利用F2群體中隱性極端個體將HTR定位在第5染色體長臂ttr-1和ttr-2兩個分子標記之間,物理距離大約在144 kb范圍內(nèi)。測序結(jié)果發(fā)現(xiàn)htr中編碼AP2轉(zhuǎn)錄因子(AP2-like ethylene-responsive transcription factor)基因發(fā)生點突變,導致保守氨基酸發(fā)生替換,亞細胞定位顯示HTR定位于細胞核中。HTR呈現(xiàn)組成型表達,但以在莖稈中表達量最高。[結(jié)論]矮稈突變體htr突變性狀受一對編碼AP2轉(zhuǎn)錄因子的隱性單基因控制。HTR基因的克隆對培育厚壁粗稈抗倒伏水稻新品種提供了一定的理論參考。
[Abstract]:[objective] to study the phenotypic analysis and gene cloning of rice dwarf mutant htr, and to provide a new dwarf gene resource for rice breeding. [methods] the stem of dwarf mutant htr was observed by cytological observation, and the F 2 population was constructed by crossing htr with N22 'and Y9311'. [results] htr was a stably inherited dwarf mutant. Its plant height, ear length, thousand grain mass, tiller number and other traits were all decreased, but the leaves became wider and the leaves were dark green. The results showed that the cells in the longitudinal axis of the stem were not normally elongated, and the number of the transverse cells was increased, compared with 9311htr in the vertical axis of the stem, and the number of the cells in the lateral direction was increased, compared with that in the vertical axis of the stem. Genetic analysis showed that the dwarf traits of the mutant htr were controlled by a pair of recessive single genes. HTR was located between the long arm ttr-1 and ttr-2 markers of chromosome 5 by using recessive extreme individuals in F2 population. The physical distance was about 144kb. The results of sequencing showed that the gene encoding AP2 transcription factor AP2-like ethylene-responsive transcription factor occurred point mutation in htr, resulting in the substitution of conserved amino acids, and subcellular localization showed that HTR was localized in the nucleus. [conclusion] the cloning of htr gene controlled by a pair of recessive single genes encoding AP2 transcription factors may provide a theoretical reference for the cultivation of new rice varieties with thick stem resistance to lodging.
【作者單位】： 南京農(nóng)業(yè)大學作物遺傳與種質(zhì)創(chuàng)新國家重點實驗室;
【基金】：國家重點研發(fā)項目七大農(nóng)作物育種專項(2016YFD0100101-08) 國家863計劃項目(2014AA10A603-15) 江蘇省科技支撐計劃項目(BE2014394,BE2015363) 江蘇省農(nóng)業(yè)科技自主創(chuàng)新資金課題(CX(16)1029)
【分類號】：S511

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相關(guān)期刊論文 前7條

1 蔣悅;孫娟;韓思迪;范磊;許凱文;江玲;王春明;;水稻黃葉突變體yl的遺傳分析與基因定位[J];南京農(nóng)業(yè)大學學報;2016年06期

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本文編號：1552781