本文關(guān)鍵詞： 自然殺傷細(xì)胞 急性移植物抗宿主病 異基因造血干細(xì)胞移植　出處：《中國實(shí)驗(yàn)血液學(xué)雜志》2017年02期 　論文類型：期刊論文

【摘要】：目的:探討異基因造血干細(xì)胞移植(allo-HSCT)后NK細(xì)胞重建與急性移植物抗宿主病(aGVHD)發(fā)生及預(yù)后的相關(guān)性。方法:采用流式細(xì)胞術(shù)檢測患者移植后30 d外周血中NK細(xì)胞的計(jì)數(shù)及活性,結(jié)合臨床表現(xiàn)和相關(guān)病理指標(biāo)綜合評價(jià)患者aGVHD發(fā)病情況,分析NK細(xì)胞計(jì)數(shù)和活性與aGVHD發(fā)生及預(yù)后的相關(guān)性。結(jié)果:移植后發(fā)生aGVHD患者的NK細(xì)胞計(jì)數(shù)和活性與未發(fā)生aGVHD患者相比較顯著降低,即655±216 cells/μl vs 1169±372 cells/μl(P=0.002)和7.3±3.6%vs 9.0±3.6%(P=0.008);發(fā)生Ⅱ-Ⅳ度aGVHD的患者與發(fā)生0-Ⅰ度aGVHD的患者相比,NK細(xì)胞計(jì)數(shù)和活性分別為617±220 cells/μl vs 1081±399 cells/μl(P=0.001)和4.2±1.7%vs 8.3±3.5%(P=0.001)。NK細(xì)胞計(jì)數(shù)和活性與aGVHD發(fā)病呈中度負(fù)相關(guān)。生存分析結(jié)果顯示,Ⅱ-Ⅳ度aGVHD組復(fù)發(fā)率高(57%vs 5%)(P=0.010),1年無進(jìn)展生存(PFS)率顯著下降(43%vs 84%)(P=0.010)。結(jié)論:移植后NK細(xì)胞計(jì)數(shù)和活性與aGVHD的發(fā)生及預(yù)后存在一定相關(guān)性,提示通過監(jiān)測NK細(xì)胞計(jì)數(shù)和活性可早期識別和預(yù)測aGVHD高�；颊�,也可能為aGVHD的防治提供了新思路。
[Abstract]:Objective: to investigate the relationship between NK cell reconstruction and the occurrence and prognosis of acute graft-versus-host disease (AGV HDD) after allo-HSCT. Methods: the count and activity of NK cells in peripheral blood of patients with allogeneic hematopoietic stem cell transplantation 30 days after transplantation were detected by flow cytometry. To evaluate the incidence of aGVHD combined with clinical manifestations and related pathological indexes. Results: NK cell count and activity in patients with aGVHD after transplantation were significantly lower than those in patients without aGVHD. 655 鹵216 cells / 渭 l vs 1169 鹵372 cells / 渭 l P0. 002) and 7.3 鹵3. 6 vs 9. 0 鹵3. 6 P0. 008, NK cell count and activity were 617 鹵220 cells / 渭 l vs 1 081 鹵399 cells / 渭 l vs 1 081 鹵399 cells / 渭 L P0. 001) and 4.2 鹵1. 7 vs 8.3 鹵3. 5T 0.001. NK cell count and activity were negatively correlated with the incidence of aGVHD. The recurrence rate of 鈪，

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