本文關(guān)鍵詞： 核盤菌 α-甘露糖苷酶基因 過表達(dá)質(zhì)粒 油菜轉(zhuǎn)化　出處：《分子植物育種》2017年01期 　論文類型：期刊論文

【摘要】：菌核病是由核盤菌引起的一種世界性的真菌病害,對(duì)我國(guó)油菜生產(chǎn)危害尤為嚴(yán)重。但是現(xiàn)在已知的所有植物資源中都找不到對(duì)核盤菌完全具有抗性的基因資源。本研究采用"以毒攻毒"的途徑,將核盤菌自身參與致病的一個(gè)α-甘露糖苷酶SSc4基因轉(zhuǎn)到甘藍(lán)型油菜中,以期獲得對(duì)能對(duì)核盤菌產(chǎn)生抗性的油菜種質(zhì)新資源。本研究克隆核盤菌的SSc4基因,構(gòu)建了其過表達(dá)質(zhì)粒,并通過農(nóng)桿菌介導(dǎo)轉(zhuǎn)化油菜,實(shí)驗(yàn)共篩選到159株抗性苗,其中獲得了27株轉(zhuǎn)基因油菜,轉(zhuǎn)化率約為1%。對(duì)其中的9株轉(zhuǎn)基因苗進(jìn)一步作RT-PCR檢測(cè),顯示有6株轉(zhuǎn)基因植株中SSc4基因高表達(dá),實(shí)現(xiàn)了設(shè)計(jì)目標(biāo)。
[Abstract]:Sclerotinia sclerotiorum is a worldwide fungal disease caused by Sclerotinia sclerotiorum. The damage to rapeseed production is especially serious in China. However, no genetic resources with complete resistance to Sclerotinia sclerotiorum can be found in all known plant resources. A 偽 -mannosidase SSc4 gene of Sclerotinia sclerotiorum was transferred to Brassica napus in order to obtain a new germplasm resource resistant to Sclerotinia sclerotiorum. In this study, the SSc4 gene of Sclerotinia sclerotiorum was cloned and its overexpression plasmid was constructed. A total of 159 resistant plants were screened by Agrobacterium tumefaciens, among which 27 transgenic rapeseed were obtained, and the transformation rate was about 1. 9 transgenic plants were further detected by RT-PCR. The results showed that there were 6 transgenic plants with high expression of SSc4 gene, which achieved the design goal.
【作者單位】： 湖南農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：國(guó)家支撐計(jì)劃項(xiàng)目(2013BAD01B03-10)資助
【分類號(hào)】：Q943.2;S435.654

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 王壯志,李琳,史耕先,劉音,趙方萄,朱立平;6A8α-甘露糖苷酶酶解p-nitrophenyl-α-D-mannopyranoside[J];中國(guó)醫(yī)學(xué)科學(xué)院學(xué)報(bào);2001年05期

2 史耕先,馬鳳蓉,朱立平,張立新,趙方萄,劉音,王訊;6A8 cDNA編碼一種α-甘露糖苷酶[J];中國(guó)醫(yī)學(xué)科學(xué)院學(xué)報(bào);1999年06期

3 趙方萄,李w，

本文編號(hào)：1524568