本文關鍵詞： 視黃醇結合蛋白基因 多態(tài)性 超重肥胖 向心性肥胖 胰島素抵抗　出處：《中國老年學雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的利用聚合酶鏈式反應(PCR)-變性高效液相色譜(DHPLC)法對老年人群視黃醇結合蛋白(RBP)4基因的rs17484721和rs36035572位點進行篩查,并分析各基因型之間肥胖、胰島素及血脂的差異。方法選擇健康查體的漢族老年人304例。檢測空腹血糖(FPG)、空腹胰島素(FINS),計算胰島素抵抗指數(HOMA-IR)。采用DHPLC技術篩查RBP4基因rs17484721和rs36035572多態(tài)性位點,測序分析結果。結果兩個位點的突變存在強連鎖不平衡。TT-II組體重指數(BMI)、腰臀比明顯高于TC-ID+CC-DD組,考慮TT-II基因型的人群出現(xiàn)向心性肥胖的概率較大。結論 RBP4基因多態(tài)性可能與老年人超重肥胖相關。
[Abstract]:Objective to screen the rs17484721 and rs36035572 loci of retinol binding protein (RBP4) gene in the elderly by polymerase chain reaction (PCR) and denaturing high performance liquid chromatography (HPLC), and to analyze the obesity among different genotypes. Methods the difference of insulin and serum lipids was studied in 304 healthy subjects of Han nationality. Fasting blood glucose (FPG), fasting insulin (Fin) and insulin resistance index (HOMA-IRN) were measured. DHPLC technique was used to screen RBP4 gene rs17484721 and rs36035572 polymorphisms. Results there was strong linkage disequilibrium between the two loci. The body mass index (BMI) of TT-II group was significantly higher than that of TC-ID CC-DD group, and the WHR was significantly higher in TT-II group than in TC-ID CC-DD group. ConclusionThe RBP4 gene polymorphism may be associated with overweight and obesity in the elderly.
【作者單位】： 華北理工大學;
【基金】：河北省醫(yī)學科學研究重點課題計劃(No.20120414)
【分類號】：R589.2

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本文編號：1508526