本文關(guān)鍵詞： DAF 標(biāo)簽SNP 單核苷酸多態(tài) 胃癌 補(bǔ)體系統(tǒng)　出處：《華北理工大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的補(bǔ)體系統(tǒng)通過(guò)參與機(jī)體對(duì)抗外來(lái)物質(zhì)的炎癥反應(yīng),改變機(jī)體微環(huán)境而影響腫瘤發(fā)生。本研究旨在探討補(bǔ)體關(guān)鍵基因C3和DAF標(biāo)簽遺傳變異與胃癌發(fā)病風(fēng)險(xiǎn)的關(guān)系,為胃癌的早期發(fā)現(xiàn),易感人群篩查提供理論依據(jù)。方法研究對(duì)象來(lái)自2008年2月到2013年12月期間在華北理工大學(xué)附屬唐山市工人醫(yī)院和華北理工大學(xué)附屬唐山市人民醫(yī)院就診的500例胃癌患者,以及500例同期健康查體個(gè)體。利用Hapmap公共數(shù)據(jù)庫(kù)提供的數(shù)據(jù),運(yùn)用Haploview4.2軟件選取補(bǔ)體C3和DAF基因的標(biāo)簽單核苷酸多態(tài)(tag SNP)。使用高通量的Sequenom Mass Array方法對(duì)所有的標(biāo)簽SNP在1000例研究對(duì)象中進(jìn)行基因分型。用卡方檢驗(yàn)分析病例組和對(duì)照組年齡、性別和吸煙狀況等基本人口學(xué)資料之間的差異。用非條件Logistic回歸模型計(jì)算各遺傳變異與胃癌發(fā)病風(fēng)險(xiǎn)的OR值及其95%CI值。結(jié)果使用Haplo View軟件我們獲得了位于補(bǔ)體C3的12個(gè)tag SNP(rs220199,rs2230205,rs2241393,rs2250656,rs2277984,rs2287846,rs3745568,rs432001,rs11569523,rs379527,rs2230204,rs433594)和位于DAF基因的2個(gè)tag SNP(rs10746463,rs2564978)用于進(jìn)一步研究。DAF rs10746463 GG、GA和AA三種基因型在病例組和對(duì)照組中分別占27.0%、52.2%、20.8%和34.0%、48.4%、17.6%。Logistic回歸分析結(jié)果顯示,DAF rs10746463多態(tài)與胃癌發(fā)病風(fēng)險(xiǎn)相關(guān)。與攜帶rs10746463 GG基因型的個(gè)體相比,AA基因型的攜帶者具有較高的胃癌發(fā)病風(fēng)險(xiǎn),其OR值(95%CI)值為1.46(1.01-2.10)。與攜帶DAF rs10746463的非吸煙者相比,攜帶至少一個(gè)A等位基因的吸煙者具有較高的胃癌發(fā)病風(fēng)險(xiǎn)(OR=1.64;95%CI=1.06-2.54);而攜帶至少一個(gè)A等位基因的非吸煙者發(fā)生胃癌的風(fēng)險(xiǎn)沒(méi)有改變(OR=1.37;95%CI=0.97-1.94)。我們的數(shù)據(jù)并沒(méi)有發(fā)現(xiàn)DAF rs2564978基因多態(tài)以及C3 tag SNP與胃癌遺傳易感性之間的關(guān)系。結(jié)論在中國(guó)北方人群中,DAF rs10746463遺傳變異影響胃癌發(fā)病風(fēng)險(xiǎn),而且該遺傳變異和吸煙存在交互作用共同增加胃癌發(fā)病風(fēng)險(xiǎn)。
[Abstract]:Objective to investigate the relationship between the genetic variation of complement key genes C3 and DAF and the risk of gastric cancer. For early detection of gastric cancer, Methods from February 2008 to December 2013, 500 patients with gastric cancer were selected from the Tangshan Workers Hospital affiliated to North China University of Technology and the people's Hospital of Tangshan City, affiliated to North China University of Technology. Using data from the Hapmap public database, The single nucleotide polymorphism of complement C3 and DAF gene was selected by Haploview4.2 software. All tagged SNP was genotyped in 1000 subjects by high-throughput Sequenom Mass Array method. The age of case group and control group were analyzed by chi-square test. The difference between basic demographic data such as sex and smoking status. The OR value and 95 CI value of each genetic variation and gastric cancer risk were calculated by non-conditional Logistic regression model. Results using Haplo View software, we obtained the complement C3. Twelve tag SNPRs220199rs2230205rs2241393rs2277984rs228784rs3745568rs432001rs11569523rs3727rs2230204rs433594) and two tag SNPRs10746463rs2564978) were used to further study the risk of gastric cancer. Compared with individuals with rs10746463 GG genotype, carriers with AA genotype had a higher risk of gastric cancer. Its OR value was 1.46 ~ 1.01-2.100.Compared with non-smokers carrying DAF rs10746463, Smokers with at least one A allele had a higher risk of developing gastric cancer, OR1.64 / 95 CI 1.06-2.54, while non-smokers with at least one A allele had no change in the risk of developing gastric cancer, OR1.3795CI0.97-1.94. Our data did not find the DAF rs2564978 gene. The relationship between polymorphism and C 3 tag SNP and the genetic susceptibility of gastric cancer. Conclusion the genetic variation of rs10746463 in the population of North China has an effect on the risk of gastric cancer. In addition, the genetic variation and smoking have an interaction to increase the risk of gastric cancer.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R735.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 許家磊;王宇;后猛;李強(qiáng);;SNP檢測(cè)方法的研究進(jìn)展[J];分子植物育種;2015年02期

2 白雪;黃可欣;馬洪喜;李銳;;卵巢上皮性癌組織中ALDH1和CD55蛋白的表達(dá)及其臨床意義[J];中國(guó)婦幼保健;2013年22期

3 楊超;李杰;劉運(yùn)江;;乳腺癌組織中CD55的表達(dá)及其與預(yù)后的關(guān)系[J];中國(guó)老年學(xué)雜志;2013年09期

4 唐立群;肖層林;王偉平;;SNP分子標(biāo)記的研究及其應(yīng)用進(jìn)展[J];中國(guó)農(nóng)學(xué)通報(bào);2012年12期

5 王芹;張玲;張維東;魏麗;賈青;;胃癌組織衰變加速因子表達(dá)及其臨床意義的研究[J];中華腫瘤防治雜志;2008年09期

6 劉勇,李啟明,路名芝,王夷黎,胡志坤;nm23-H1、p53、PCNA表達(dá)與大腸癌浸潤(rùn)轉(zhuǎn)移的關(guān)系[J];臨床與實(shí)驗(yàn)病理學(xué)雜志;1997年01期

，

本文編號(hào)：1497813