本文關鍵詞： 水稻 葉片卷曲 LBD基因家族 泡狀細胞　出處：《中國農業(yè)科學院》2016年碩士論文　論文類型：學位論文

【摘要】：水稻葉片是光合作用、呼吸作用、蒸騰作用的重要場所,是決定水稻產(chǎn)量的重要農藝性狀之一。葉片適度卷曲有利于葉片挺直,改善披垂狀態(tài),增加光合作用和水稻單產(chǎn)。泡狀細胞是與卷葉性狀相關的位于葉片近軸面的薄壁細胞,當植物處于干旱環(huán)境時,泡狀細胞失水,導致葉片近軸卷曲。本研究發(fā)現(xiàn)了一個新LBD家族基因OsLBD3-7可以通過調節(jié)泡狀細胞,影響葉片卷曲。LBD基因家族是植物特異的一類轉錄因子,含有一個由與DNA元件相互作用的半胱氨酸保守結構域、GAS保守結構域和與蛋白質相互作用的羧基端亮氨酸保守結構構成的LOB保守結構域,參與側生器官原基的啟動和形態(tài)建成,對植物特定器官的形成與發(fā)育具有重要影響。研究發(fā)現(xiàn):OsLBD3-7融合VP64形成的人工合成轉錄因子OsLBD3-7V和OsLBD3-7的簡單過表達轉基因植株均呈現(xiàn)劍葉寬度減少和近軸卷曲表型;葉片橫截面細胞學切片分析表明,泡狀細胞數(shù)目和面積減少導致葉片近軸卷曲。利用定量PCR檢測了OsLBD3-7表達譜,發(fā)現(xiàn)該基因在所有組織器官中都有表達,尤其在根和鞘組織中表達量較高;亞細胞定位結果表明定位于細胞核中和細胞膜上。酵母自激活活性結果表明OsLBD3-7為弱轉錄激活子。進一步在OsLBD3-7V和OsLBD3-7轉基因植物中對泡狀細胞調節(jié)基因檢測結果顯示,泡狀細胞負調節(jié)基因表達量升高,而正調節(jié)基因表達量降低,表明OsLBD3-7作為泡狀細胞上游負調節(jié)子參與葉片卷葉調控。
[Abstract]:Rice leaf is an important place for photosynthesis, respiration and transpiration, which is one of the important agronomic traits that determine rice yield. Increased photosynthesis and rice yield. Vesicular cells are paraxial parenchyma cells associated with leaf curl traits. When plants are in dry conditions, bubbly cells lose water. In this study, we found that a new LBD family gene OsLBD3-7 can affect leaf curl. LBD gene family is a kind of plant specific transcription factors by regulating vesicular cells. A conserved LOB domain consisting of a cysteine conserved domain interacting with DNA elements and a carboxyl-terminal leucine conserved domain interacting with proteins is involved in the initiation and morphogenesis of the primordium of the lateral organ. It was found that the simple overexpression of OsLBD3-7V and OsLBD3-7, a synthetic transcription factor formed by the fusion of VP64, showed a reduced leaf width and a coiled phenotype in the near axis of the transgenic plants, which had a significant effect on the formation and development of specific organs in plants. The cytological analysis of leaf cross-section showed that the decrease of the number and area of vesicular cells resulted in leaf coiling. The expression profile of OsLBD3-7 was detected by quantitative PCR, and it was found that the gene was expressed in all tissues and organs. Especially in the root and sheath tissue, the expression level was higher. The results of subcellular localization showed that it was located in the nucleus and the cell membrane. The self-activation activity of yeast indicated that OsLBD3-7 was a weak transcriptional activator. Furthermore, in OsLBD3-7V and OsLBD3-7 transgenic plants, the regulatory genes of vesicular cells were detected. The expression of negative regulatory genes increased in vesicular cells, but decreased in positive ones, indicating that OsLBD3-7, as a negative regulator upstream of bubbly cells, was involved in leaf coiling regulation.
【學位授予單位】：中國農業(yè)科學院
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S511

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本文編號：1494456