本文關(guān)鍵詞： 水稻 葉片卷曲 LBD基因家族 泡狀細(xì)胞　出處：《中國農(nóng)業(yè)科學(xué)院》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：水稻葉片是光合作用、呼吸作用、蒸騰作用的重要場所,是決定水稻產(chǎn)量的重要農(nóng)藝性狀之一。葉片適度卷曲有利于葉片挺直,改善披垂?fàn)顟B(tài),增加光合作用和水稻單產(chǎn)。泡狀細(xì)胞是與卷葉性狀相關(guān)的位于葉片近軸面的薄壁細(xì)胞,當(dāng)植物處于干旱環(huán)境時,泡狀細(xì)胞失水,導(dǎo)致葉片近軸卷曲。本研究發(fā)現(xiàn)了一個新LBD家族基因OsLBD3-7可以通過調(diào)節(jié)泡狀細(xì)胞,影響葉片卷曲。LBD基因家族是植物特異的一類轉(zhuǎn)錄因子,含有一個由與DNA元件相互作用的半胱氨酸保守結(jié)構(gòu)域、GAS保守結(jié)構(gòu)域和與蛋白質(zhì)相互作用的羧基端亮氨酸保守結(jié)構(gòu)構(gòu)成的LOB保守結(jié)構(gòu)域,參與側(cè)生器官原基的啟動和形態(tài)建成,對植物特定器官的形成與發(fā)育具有重要影響。研究發(fā)現(xiàn):OsLBD3-7融合VP64形成的人工合成轉(zhuǎn)錄因子OsLBD3-7V和OsLBD3-7的簡單過表達(dá)轉(zhuǎn)基因植株均呈現(xiàn)劍葉寬度減少和近軸卷曲表型;葉片橫截面細(xì)胞學(xué)切片分析表明,泡狀細(xì)胞數(shù)目和面積減少導(dǎo)致葉片近軸卷曲。利用定量PCR檢測了OsLBD3-7表達(dá)譜,發(fā)現(xiàn)該基因在所有組織器官中都有表達(dá),尤其在根和鞘組織中表達(dá)量較高;亞細(xì)胞定位結(jié)果表明定位于細(xì)胞核中和細(xì)胞膜上。酵母自激活活性結(jié)果表明OsLBD3-7為弱轉(zhuǎn)錄激活子。進(jìn)一步在OsLBD3-7V和OsLBD3-7轉(zhuǎn)基因植物中對泡狀細(xì)胞調(diào)節(jié)基因檢測結(jié)果顯示,泡狀細(xì)胞負(fù)調(diào)節(jié)基因表達(dá)量升高,而正調(diào)節(jié)基因表達(dá)量降低,表明OsLBD3-7作為泡狀細(xì)胞上游負(fù)調(diào)節(jié)子參與葉片卷葉調(diào)控。
[Abstract]:Rice leaf is an important place for photosynthesis, respiration and transpiration, which is one of the important agronomic traits that determine rice yield. Increased photosynthesis and rice yield. Vesicular cells are paraxial parenchyma cells associated with leaf curl traits. When plants are in dry conditions, bubbly cells lose water. In this study, we found that a new LBD family gene OsLBD3-7 can affect leaf curl. LBD gene family is a kind of plant specific transcription factors by regulating vesicular cells. A conserved LOB domain consisting of a cysteine conserved domain interacting with DNA elements and a carboxyl-terminal leucine conserved domain interacting with proteins is involved in the initiation and morphogenesis of the primordium of the lateral organ. It was found that the simple overexpression of OsLBD3-7V and OsLBD3-7, a synthetic transcription factor formed by the fusion of VP64, showed a reduced leaf width and a coiled phenotype in the near axis of the transgenic plants, which had a significant effect on the formation and development of specific organs in plants. The cytological analysis of leaf cross-section showed that the decrease of the number and area of vesicular cells resulted in leaf coiling. The expression profile of OsLBD3-7 was detected by quantitative PCR, and it was found that the gene was expressed in all tissues and organs. Especially in the root and sheath tissue, the expression level was higher. The results of subcellular localization showed that it was located in the nucleus and the cell membrane. The self-activation activity of yeast indicated that OsLBD3-7 was a weak transcriptional activator. Furthermore, in OsLBD3-7V and OsLBD3-7 transgenic plants, the regulatory genes of vesicular cells were detected. The expression of negative regulatory genes increased in vesicular cells, but decreased in positive ones, indicating that OsLBD3-7, as a negative regulator upstream of bubbly cells, was involved in leaf coiling regulation.
【學(xué)位授予單位】：中國農(nóng)業(yè)科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S511

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 ZHAO Tao;LIU Jun;LI Hong-Yu;LIN Jian-Zhong;BIAN Ming-Di;ZHANG Chun-Yu;ZHANG Yong-Xing;PENG Yu-Chong;LIU Bin;LIN ChenTao;;Using hybrid transcription factors to study gene function in rice[J];Science China(Life Sciences);2015年11期

2 ;Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis[J];Cell Research;2000年04期

，

本文編號：1494456