本文關(guān)鍵詞： 小麥 TaZTP基因 耐鹽 基因表達(dá) 亞細(xì)胞定位　出處：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2017年10期 　論文類型：期刊論文

【摘要】：土壤鹽堿化是小麥(Triticum aestivum)生產(chǎn)的主要限制因子之一。鋅轉(zhuǎn)運(yùn)體(zinc transporter,ZTP)屬于鋅鐵調(diào)控蛋白(ZRT,IRT-like protein,ZIP)蛋白家族,并參與調(diào)控非折疊蛋白應(yīng)激響應(yīng),是一個優(yōu)良的抗鹽基因。本研究采用同源克隆的方法獲得了ZTP29在小麥中的直系同源基因TaZTP29,利用生物信息學(xué)、qRT-PCR、綠色熒光蛋白示蹤技術(shù)和轉(zhuǎn)基因擬南芥(Arabidopsis thaliana)過表達(dá)的方法明確了TaZTP29的保守域信息、表達(dá)模式、亞細(xì)胞定位信息和功能。序列分析結(jié)果表明,TaZTP29基因(GenBank登錄號:KY610283)含有81 bp的5'UTR、834 bp基因編碼區(qū)及117 bp的3'UTR,編碼277個氨基酸。TaZTP29蛋白具有典型的ZIP保守域,具有8個跨膜域,且在第V跨膜域具有完全保守的組氨酸蛋白殘基,定位于質(zhì)膜上,屬于ZIP蛋白家族。序列同源性和進(jìn)化分析表明,TaZTP29與節(jié)節(jié)麥(Aegilops tauschii)ZIP29蛋白同源性最高;與擬南芥(Arabidopsis thaliana)ZTP29蛋白同源性最遠(yuǎn)。TaZTP29強(qiáng)烈響應(yīng)鋅和鹽脅迫,上調(diào)表達(dá),在高溫與干旱脅迫下,下調(diào)表達(dá);且TaZTP29主要在地下部分的根中表達(dá),在地上部分的組織中表達(dá)量較低�？果}性鑒定表明,在不加NaCl的條件下,野生型與過表達(dá)株系總根長基本一致,在100和150 mmol/L NaCl處理的培養(yǎng)基中,3個轉(zhuǎn)基因株系的總跟長顯著長于野生型擬南芥,表明過表達(dá)株系對NaCl脅迫具有較好的耐性。轉(zhuǎn)入TaZTP29后,在擬南芥中過表達(dá)能夠提高擬南芥對鹽脅迫的抗性,TaZTP29具有抗鹽功能,為進(jìn)一步改良小麥抗逆性等分子機(jī)理研究提供了新的依據(jù)。
[Abstract]:Soil salinization is one of the main limiting factors for Triticum aestivum production. ZTP belongs to the ZIP family of zinc-ferritin regulatory protein ZRTT-like protein and participates in the regulation of non-folding protein stress response. TaZTP29, a homologous gene of ZTP29 in wheat, was obtained by homologous cloning and bioinformatics qRT-PCR. The green fluorescent protein tracing technique and the method of overexpression of Arabidopsis thaliana in transgenic Arabidopsis confirmed the conserved domain information and expression pattern of TaZTP29. Subcellular location information and function. Sequence analysis showed that TaZTP29 gene (GenBank accession number: KY610283) contained 81bp 5UUTR. The coding region of 834bp gene and the 117bp 3G UTRs encode 277 amino acids. TaZTP29 protein has typical conserved ZIP domain and 8 transmembrane domains. And in the V transmembrane domain there is a completely conserved histidine protein residues located on the plasma membrane belonging to the ZIP protein family. Sequence homology and evolution analysis showed. TaZTP29 had the highest homology with Aegilops tauschii)ZIP29. TaZTP29, the farthest homology of Arabidopsis thaliana)ZTP29 protein from Arabidopsis, strongly responded to zinc and salt stress and upregulated the expression. Under high temperature and drought stress, the expression was down-regulated. The expression of TaZTP29 was mainly in the roots of the underground part, but low in the tissue of the aboveground part. The salt tolerance test showed that the expression of TaZTP29 was not under the condition of NaCl. The total root length of wild type and over-expressed strain was basically the same. In the medium treated with 100 and 150 mmol/L NaCl, the total heel length of three transgenic lines was significantly longer than that of wild type Arabidopsis thaliana. The results showed that the overexpression lines had better tolerance to NaCl stress, and overexpression in Arabidopsis thaliana could enhance the resistance of Arabidopsis thaliana to salt stress, TaZTP29 had the function of salt resistance. It provides a new basis for the further improvement of wheat stress resistance and other molecular mechanisms.
【作者單位】： 西北農(nóng)林科技大學(xué)農(nóng)學(xué)院;
【基金】：國家科技支撐計(jì)劃項(xiàng)目(No.2013BAD04B02) 陜西省科技統(tǒng)籌創(chuàng)新項(xiàng)目(No.2014KZTB02-01-01) 中央高�；究蒲袠I(yè)務(wù)費(fèi)(No.Z109021423)
【分類號】：Q943.2;S512.1
【正文快照】： 小麥(Triticum aestivum)是我國第二大糧食作物,小麥的安全生產(chǎn)對保障我國的糧食安全具有重要意義(Zhang et al.,2016)。高溫、干旱,特別是鹽漬等非生物逆境已成為小麥產(chǎn)量和品質(zhì)提高的主要限制因素。闡明植物高鹽脅迫應(yīng)答信號傳導(dǎo)通路、克隆信號通路中的關(guān)鍵基因是利用生物技，

本文編號：1486965