本文關鍵詞： 大麥 GS基因 相對表達量 氮高效利用　出處：《核農學報》2017年07期 　論文類型：期刊論文

【摘要】：為探明大麥品種的不同氮利用效率差異及質體型谷氨酰胺合成酶基因的表達差異,以48份大麥品種為材料,并利用不同氮水平營養(yǎng)液水培8周,研究其在低氮(0.4 mmol·L~(-1))、中氮(2.0 mmol·L~(-1))及高氮(5.0 mmol·L~(-1))下根、莖、葉的相關性狀,篩選氮高效利用和氮低效利用的大麥品種,并利用qRT-PCR對篩選到的大麥氮高效和氮低效品種的GS2基因表達特性進行分析。結果表明,中氮下,除根干重和根含氮量外,其余的大麥苗期根、莖、葉各生物性狀在品種間差異極顯著,根據不同氮處理下的48份大麥品種苗期性狀以及氮利用效率,篩選到1個氮高效品種(Z0099001)和2個氮低效品種(ARr-91和甘啤7號)。3個氮處理水平下,氮高效品種GS2基因的相對表達量高于氮低效品種,并且氮高效品種和氮低效品種根、莖、葉中GS2基因的相對表達量也各不相同,其中,葉中最高,莖中次之,根中最低。低氮條件下,根莖葉中,Z0099001的GS2基因的相對表達量高于甘啤7號,ARr-91最低。中氮條件下,根、莖、葉中均表現為氮高效品種高于氮低效品種,但在根中差異不顯著,莖和葉中差異顯著。高氮條件下,3個品種在根中GS2基因的相對表達量較低;在莖和葉中,Z0099001的相對表達量顯著高于ARr-91和甘啤7號,且Z0099001葉中GS2基因的相對表達量在所有性狀中最高。本研究結果為進一步解析大麥氮高效利用率奠定了基礎。
[Abstract]:In order to find out the difference of nitrogen use efficiency and the expression of glutamine synthase gene in barley varieties, 48 barley varieties were cultured in water for 8 weeks with different nitrogen levels. The root and stem were studied at 0. 4 mmol 路L ~ (-1) of low nitrogen, 2.0 mmol 路L ~ (-1) of medium nitrogen and 5.0 mmol 路L ~ (-1) of high nitrogen. Leaf correlation traits were used to screen barley varieties with high nitrogen efficiency and low nitrogen efficiency. QRT-PCR was used to analyze the characteristics of GS2 gene expression in the selected barley varieties with high nitrogen efficiency and low nitrogen efficiency. The results showed that the roots of barley at seedling stage except root dry weight and root nitrogen content under medium nitrogen. The traits of stem and leaf were significantly different among varieties. According to the seedling characters and nitrogen utilization efficiency of 48 barley varieties treated with different nitrogen treatments. One nitrogen efficient cultivar (Z0099001) and two low nitrogen efficient varieties (Z0099001) and 2 low nitrogen varieties (ZARr-91 and Gan Beer No. 7) were selected under three nitrogen treatments. The relative expression amount of GS2 gene in nitrogen efficient variety was higher than that in low efficiency nitrogen variety, and the relative expression amount of GS2 gene in root, stem and leaf of nitrogen efficient variety and low nitrogen efficient variety were different, among which, the highest was in leaf. The relative expression of Z0099001 GS2 gene in rhizome leaves was higher than that in Gan Beer No. 7 ARr-91. Under medium nitrogen condition, root and stem were the lowest. The results showed that all the varieties with high nitrogen efficiency were higher than those with low nitrogen efficiency, but there was no significant difference in root, but there were significant differences between stems and leaves. Under the condition of high nitrogen, the relative expression of GS2 gene was lower in the root of the three varieties. The relative expression of Z0099001 in stem and leaf was significantly higher than that in ARr-91 and Gan Beer 7. The relative expression of GS2 gene in leaves of Z0099001 was the highest among all traits. The results laid a foundation for further analysis of nitrogen utilization efficiency in barley.
【作者單位】： 甘肅農業(yè)大學/甘肅省作物遺傳改良與種質創(chuàng)新重點實驗室/甘肅省干旱生境作物學重點實驗室;甘肅農業(yè)大學農學院;甘肅農業(yè)大學生命科學技術學院;
【基金】：國家自然基金(31171558,31460347) 農業(yè)產業(yè)技術體系建設項目(國家大麥青稞產業(yè)體系,CARS-05) 盛彤笙創(chuàng)新基金(GSAU-STS-1513)
【分類號】：Q943.2;S512.3
【正文快照】： 大麥(Hordeum vulgare L.)是世界上重要的糧食作物,也是生產啤酒的主要原料[1],其具有早熟、耐旱、耐瘠和耐鹽等特點,在世界各地廣泛種植[2]。氮是影響植物產量的最敏感元素,氮素吸收與積累是產量形成的重要基礎,但是氮肥的過量使用會造成其利用率降低,增加氮素資源的無效浪費

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