發(fā)布時(shí)間：2018-01-20 13:42

  本文關(guān)鍵詞： 家兔 AANAT 單核苷酸基因多態(tài)性 生長性狀　出處：《中國畜牧雜志》2017年10期 　論文類型：期刊論文

【摘要】：為研究五羥色胺-N-乙�；D(zhuǎn)移酶(AANAT)基因編碼區(qū)(CDS區(qū))多態(tài)性和家兔生長性狀的關(guān)聯(lián)性,以天府黑兔、伊拉兔和新西蘭兔作為實(shí)驗(yàn)材料,對(duì)AANAT基因的外顯子進(jìn)行PCR擴(kuò)增,直接測序檢測家兔AANAT基因外顯子的單核苷酸多態(tài)性。結(jié)果表明:在3個(gè)兔品種的CDS區(qū)上均發(fā)現(xiàn)有5個(gè)完全連鎖突變位點(diǎn),分別是C.132TC、C.153TC、C.159 AT、C.234 AG、C.291 AG,形成了野生型TTTTAAAAAA、雜合型TCTCATAGAG、突變型CCCCTTGGGG 3種基因型,突變型新西蘭兔在56、70、84日齡的體重顯著高于野生型和雜合型(P0.05),而突變型伊拉兔和天府黑兔在70、84日齡的體重顯著高于雜合型和野生型(P0.05)。綜上可知,AANAT基因可作為肉兔生長性狀的候選基因。
[Abstract]:In order to study the association between polymorphism of serotonin N-acetyltransferase (AANATE) gene coding region and growth traits of rabbits, Tianfu black rabbit, Ira rabbit and New Zealand rabbit were used as experimental materials. The exon of AANAT gene was amplified by PCR. The single nucleotide polymorphisms of the exon of AANAT gene in rabbits were detected by direct sequencing. The results showed that there were five completely linked mutation sites in the CDS region of the three rabbits, namely C.132TC. The wild type TTTTAAAA, heterozygote TCTCATAGAG was formed. The body weight of mutant New Zealand rabbits was significantly higher than that of wild type and heterozygote (P0.05). However, the body weight of mutant Ira rabbits and Tianfu black rabbits was significantly higher than that of heterozygote and wild type P0.05N at 70 ~ 84 days old, which suggested that AANAT gene could be used as a candidate gene for growth traits of meat rabbits.
【作者單位】： 四川農(nóng)業(yè)大學(xué)動(dòng)物遺傳育種研究所;
【基金】：四川省科技支撐計(jì)劃項(xiàng)目(2016NYZ0046、2016NYZ0050)
【分類號(hào)】：Q78;S829.1
【正文快照】： 我國是養(yǎng)兔大國,也是兔肉出口大國。家兔是一種非常重要的經(jīng)濟(jì)動(dòng)物,它既可以食用,也可以作為工業(yè)原料、觀賞動(dòng)物、醫(yī)用模型等。五羥色胺-N-乙�；D(zhuǎn)移酶(AANAT)是褪黑激素(Melatonin,MT)合成過程的限速酶[1]。MT在生物體內(nèi)具有調(diào)控動(dòng)物的生長、應(yīng)激、炎癥、晝夜節(jié)律、視網(wǎng)膜信

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