發(fā)布時(shí)間：2018-01-14 02:19

  本文關(guān)鍵詞：香樟兩個(gè)DREB1轉(zhuǎn)錄因子基因的分離及其對(duì)不同脅迫的響應(yīng)分析　出處：《園藝學(xué)報(bào)》2016年04期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 香樟 CBF/DREB 基因克隆 表達(dá)分析

【摘要】：以香樟(Cinnamomum camphora)實(shí)生苗為試驗(yàn)材料,利用同源克隆結(jié)合RACE技術(shù)獲得了兩個(gè)冷誘導(dǎo)轉(zhuǎn)錄因子基因CBF/DREB1的cDNA全長(zhǎng)序列,命名為CcCBFc和CcCBFd,Gen Bank登錄號(hào)分別為KP336741和KP336742。序列分析顯示這兩個(gè)基因均沒(méi)有內(nèi)含子,cDNA全長(zhǎng)為897和1 010 bp,開(kāi)放閱讀框分別為654和621 bp,編碼217和206個(gè)氨基酸,預(yù)測(cè)蛋白分子量分別為24.0和22.9 kD,等電點(diǎn)分別為5.26和8.58�；诎被嵝蛄械耐葱员葘�(duì)和系統(tǒng)進(jìn)化樹(shù)分析表明,這兩個(gè)基因均屬于DREB1家族,與雙子葉植物進(jìn)化關(guān)系較近。實(shí)時(shí)定量PCR結(jié)果顯示,CcCBFc和CcCBFd都能被低溫(4℃)、干旱(20%PEG)、鹽(250 mmol·L~(-1) NaCl)和ABA(100μmol·L~(-1))誘導(dǎo),表明CcCBFc和CcCBFd可能在香樟應(yīng)對(duì)非生物脅迫過(guò)程中發(fā)揮重要作用。
[Abstract]:The seedling of Cinnamomum camphora was used as the experimental material. The full-length cDNA sequences of two cold-induced transcription factor gene CBF/DREB1 were obtained by homologous cloning and RACE technique. They were named CcCBFc and CcCBFd. The accession numbers of Gen Bank were KP336741 and KP336742.The sequence analysis showed that the length of Gen Bank was 897 BP and 1 010 BP respectively. The open reading frames were 654 and 621 BP, encoding 217 and 206 amino acids, respectively. The predicted molecular weights of the predicted proteins were 24.0 and 22.9 KD, respectively. The isoelectric points were 5.26 and 8.58 respectively. The homologous alignment based on amino acid sequence and phylogenetic tree analysis showed that the two genes belonged to the DREB1 family. The results of real-time quantitative PCR showed that both CcCBFc and CcCBFd could be treated at 4 鈩，

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