發(fā)布時(shí)間：2018-01-04 23:30

  本文關(guān)鍵詞：不同環(huán)境因子對(duì)海洋細(xì)菌Pseudoalteromonas issachenkoniiHZ引導(dǎo)糖基轉(zhuǎn)移酶基因表達(dá)的影響　出處：《食品工業(yè)科技》2016年22期 　論文類型：期刊論文

  更多相關(guān)文章： 海洋細(xì)菌Pseudoalteromonas issachenkonii HZ 引導(dǎo)糖基轉(zhuǎn)移酶 實(shí)時(shí)熒光定量PCR

【摘要】：為了研究海洋細(xì)菌Pseudoalteromonas issachenkonii HZ所產(chǎn)多糖的生物合成基因簇,首先需要克隆到在多糖合成中起關(guān)鍵作用的引導(dǎo)糖基轉(zhuǎn)移酶基因。通過設(shè)計(jì)引物,采用PCR技術(shù)從海洋細(xì)菌Pseudoalteromonas issachenkonii HZ成功克隆到該基因,并通過實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)了溫度、p H及海鹽濃度對(duì)引導(dǎo)糖基轉(zhuǎn)移酶基因表達(dá)量的影響。結(jié)果表明:低溫有利于引導(dǎo)糖基轉(zhuǎn)移酶的表達(dá),隨著溫度的上升,引導(dǎo)糖基轉(zhuǎn)移酶基因的表達(dá)量先增后減,在20 h時(shí),引導(dǎo)糖基轉(zhuǎn)移酶的表達(dá)量急劇上升,15℃和35℃引導(dǎo)糖基轉(zhuǎn)移酶表達(dá)量分別是20℃表達(dá)量的5.2倍、5.8倍;海鹽濃度為4.5%時(shí)引導(dǎo)糖基轉(zhuǎn)移酶的表達(dá)量為海鹽濃度3.5%的2.5倍,海鹽濃度為5.5%時(shí)引導(dǎo)糖基轉(zhuǎn)移酶的表達(dá)量為海鹽濃度3.5%的2.0倍;10 h時(shí)p H為8、9的培養(yǎng)基中引導(dǎo)糖基轉(zhuǎn)移酶的表達(dá)量分別為p H為7的1.64和1.67倍。該結(jié)果為探究菌體環(huán)境適應(yīng)性機(jī)制提供了一定的基礎(chǔ)。
[Abstract]:In order to study the biosynthesis gene cluster of polysaccharides produced by marine bacteria Pseudoalteromonas issachenkonii HZ. First of all, we need to clone the leading glycosyltransferase gene which plays a key role in the synthesis of polysaccharides. The gene was cloned successfully from marine bacteria Pseudoalteromonas issachenkonii HZ by PCR. The effects of temperature pH and sea salt concentration on the gene expression of guided glycosyltransferase were detected by real-time fluorescence quantitative PCR. The results showed that low temperature was beneficial to the expression of glycosyltransferase. With the increase of temperature, the expression of leading glycosyltransferase gene increased first and then decreased. At 20 h, the expression of guided glycosyltransferase increased sharply. The expression of glycosyltransferase at 15 鈩，

本文編號(hào)：1380611