本文關(guān)鍵詞：云南漢族γ-干擾素受體基因多態(tài)性與動(dòng)脈粥樣硬化斑塊穩(wěn)定性的相關(guān)性　出處：《重慶醫(yī)學(xué)》2016年33期 　論文類型：期刊論文

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【摘要】：目的探討云南漢族γ-干擾素受體(IFNGR)的兩個(gè)氨基酸位點(diǎn)Val14Met和GIn64Arg多態(tài)性與動(dòng)脈粥樣硬化(AS)斑塊穩(wěn)定性的相關(guān)性。方法收集該院2014年3月至2015年3月收治的AS斑塊不穩(wěn)定的患者作為觀察組,而同期入院的AS斑塊穩(wěn)定/無斑塊的患者為對(duì)照組。采集患者外周靜脈血,提取基因組DNA,通過聚合酶鏈反應(yīng)(PCR)產(chǎn)物直接測(cè)序法檢測(cè)IFNGR1Vall4Met和IFNGR2Gln64Arg位點(diǎn)的基因型,采用DNAStar、GeneTool軟件分析測(cè)序結(jié)果,用流式細(xì)胞術(shù)檢測(cè)患者血漿細(xì)胞因子[γ-干擾素(IFN-γ)]的水平。結(jié)果 204例當(dāng)?shù)貪h族患者列入研究,其中觀察組109例,年齡(76.89±12.08)歲;對(duì)照組95例,年齡(65.99±16.32)歲。IFNGR1Vall4Met位點(diǎn)在觀察組和對(duì)照組中均未發(fā)現(xiàn)多態(tài)性改變。IFNGR2Gln64Arg在觀察組AA基因型頻率為51.95%(40/77),AG基因型頻率為53.06%(52/98),GG基因型頻率為58.62%(17/29);對(duì)照組AA基因型頻率為48.05%(37/77),AG基因型頻率為46.94%(46/98),GG基因型頻率為41.38%(12/29),卡方檢驗(yàn)P=0.824,IFNGR2基因型AA、AG和GG與AS斑塊穩(wěn)定性沒有關(guān)系;觀察組IFNGR2A等位基因頻率為52.38%(132/252),G等位基因頻率為55.13%(86/156),對(duì)照組A等位基因頻率為47.62%(120/252),G等位基因頻率為44.87%(70/156),卡方檢驗(yàn)校正P=0.661,IFNGR2等位基因A和G與AS斑塊穩(wěn)定性沒有關(guān)系。經(jīng)Hardy-Weinberg遺傳平衡檢驗(yàn),該樣本人群該基因頻率符合遺傳平衡法則。觀察組血漿中IFN-γ水平為(4.60±1.91)ng/mL,對(duì)照組為(4.88±2.10)ng/mL,差異無統(tǒng)計(jì)學(xué)意義(P=0.318);血漿IFN-γ水平與AS斑塊穩(wěn)定性無相關(guān)性(P=0.308)。結(jié)論 IFNGR基因多態(tài)性不能作為AS斑塊穩(wěn)定性的預(yù)警指標(biāo)。
[Abstract]:Objective to investigate the Yunnan Han interferon gamma receptor (IFNGR) two amino acid sites Val14Met and GIn64Arg polymorphism and atherosclerotic plaque stability (AS) correlation. Methods in our hospital from March 2014 to March 2015 were AS plaque instability patients as the observation group, while AS admitted plaque stability / no plaque of patients as control group. Patients with peripheral venous blood collection, extract genomic DNA by polymerase chain reaction (PCR) genotype, IFNGR1Vall4Met and IFNGR2Gln64Arg were detected by DNAStar sequencing, sequencing analysis results of GeneTool software, flow cytometry was used to detect plasma cytokines in patients with [interferon gamma (IFN- y)] level. Results 204 cases of local Han patients included in the study, 109 cases in the observation group, age (76.89 + 12.08) years; 95 cases in the control group, age (65.99 + 16.32).IFNGR1Vall4Met sites in the view Observation group and control group were not found in.IFNGR2Gln64Arg polymorphism in the observation group AA genotype frequency was 51.95% (40/77), AG genotype frequency was 53.06% (52/98), GG genotype frequency was 58.62% (17/29); control group AA genotype frequency was 48.05% (37/77), AG genotype frequency was 46.94% (46/98), GG genotype frequency was 41.38% (12/29), chi square test P=0.824, IFNGR2 genotype AA, AG and GG AS and plaque stability Never mind; the observation group IFNGR2A allele frequency was 52.38% (132/252), G allele frequency was 55.13% (86/156), the control group A allele the frequency of 47.62% (120/252), G allele frequency was 44.87% (70/156), chi square test corrected P=0.661, IFNGR2 allele and A G and AS Hardy-Weinberg. The stability of plaque Never mind genetic equilibrium test, the gene frequency of the sample population with genetic equilibrium law. Observe the level of plasma for IFN- gamma (4. 60 + 1.91) ng/mL, the control group was (4.88 + 2.10) ng/mL, the difference was not statistically significant (P=0.318). There was no correlation between plasma IFN- gamma level and the stability of AS plaque (P=0.308). Conclusion IFNGR gene polymorphism can not be used as an early warning indicator for the stability of AS plaque.

【作者單位】： 云南省第一人民醫(yī)院內(nèi)干科;云南省第一人民醫(yī)院基研室;
【分類號(hào)】：R543.5
【正文快照】： 動(dòng)脈粥樣硬化(atherosclerosis,AS)是一種常見多發(fā)疾病,其斑塊的不穩(wěn)定導(dǎo)致斑塊破裂、血栓形成及血管阻塞,是急性心、腦血管事件的主要原因,極大的威脅著人民群眾的生命健康,防治AS已成為當(dāng)今醫(yī)學(xué)科學(xué)研究的重大課題。γ-干擾素(IFN-γ)是多功能細(xì)胞因子,是巨噬細(xì)胞、自然殺傷

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本文編號(hào)：1368755