本文關(guān)鍵詞：紫花苜蓿MsHST基因克隆及擬南芥轉(zhuǎn)化　出處：《草地學(xué)報(bào)》2016年06期 　論文類型：期刊論文

  更多相關(guān)文章： 紫花苜蓿 MsHST 擬南芥 轉(zhuǎn)基因

【摘要】：運(yùn)用RT-PCR法從紫花苜蓿(Medicago sativa)中成功克隆出長(zhǎng)度約1100bp的尿黑酸法呢基轉(zhuǎn)移酶基因MsHST,采用qRT-PCR的方法,分析MsHST基因的表達(dá)特征,結(jié)果顯示MsHST在所有組織中均有表達(dá),但在葉片中表達(dá)量最高,根中最低;隨葉片的衰老,表達(dá)量降低。且構(gòu)建了超表達(dá)載體pBI-MsHST,并獲得擬南芥(Arabidopsis thaliana)轉(zhuǎn)基因植株,經(jīng)PCR,RT-PCR和GUS染色檢測(cè)轉(zhuǎn)基因植株,初步證實(shí)目的基因在擬南芥中表達(dá)。
[Abstract]:By using the method of RT-PCR from Alfalfa (Medicago sativa) successfully cloned homogentisic acid method with length of about 1100bp farnesyl transferase gene MsHST, qRT-PCR method was used to analyze the features of MsHST gene expression, the results showed that MsHST in all tissues, but the highest expression level in leaves and roots with the lowest; leaf senescence, decreased expression. The overexpression vector pBI-MsHST was constructed, and the Arabidopsis thaliana transgenic plants were obtained. The transgenic plants were detected by PCR, RT-PCR and GUS staining, and preliminarily confirmed that the target gene was expressed in Arabidopsis thaliana.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;中國農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所;
【基金】：十二五國家科技支撐子課題“紫花苜蓿品質(zhì)性狀相關(guān)基因的挖掘與功能分析”(2014CB138703-2) 中國農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所基本科研業(yè)務(wù)費(fèi)項(xiàng)目(2014ywf-zd-2)資助
【分類號(hào)】：S541.9;Q943.2
【正文快照】： 尿黑酸法呢基轉(zhuǎn)移酶(Homogentisate solanesyl-transferase,HST)是高等植物體內(nèi)普遍存在的一種不可或缺的酶,他對(duì)植物生長(zhǎng)發(fā)育的調(diào)控及光系統(tǒng)II(PSII)傳遞電子的輔因子PQ的合成發(fā)揮著不可替代的重要作用[1,2]。除此之外,尿黑酸法呢基轉(zhuǎn)移酶還能參與植物代謝中類胡蘿卜素與ABA，

本文編號(hào)：1347815