本文關(guān)鍵詞：企鵝珍珠貝GLUT1基因全長cDNA克隆及其對葡萄糖的表達響應　出處：《南方水產(chǎn)科學》2016年05期 　論文類型：期刊論文

  更多相關(guān)文章： 企鵝珍珠貝 GLUT 基因克隆 葡萄糖

【摘要】：葡萄糖轉(zhuǎn)運蛋白(GLUTs)是能量代謝的關(guān)鍵因子,為了探索GLUT1(glucose transporter type1)在企鵝珍珠貝(Pteria penguin)中的功能,開展了企鵝珍珠貝GLUT1基因的克隆與功能研究。利用RACE技術(shù)克隆出企鵝珍珠貝GLUT1基因,并命名為Pp GLUT1。該基因c DNA全長為2 068 bp,編碼522個氨基酸,有2個跨膜螺旋區(qū),N端和C端都在胞質(zhì)側(cè),分子量為57.226 4 k D,理論等電點為5.17。不同組織熒光定量結(jié)果表明,在腸和外套膜中表達量最高,閉殼肌中的表達量顯著低于其他組織。0.5 g·m L-1的高質(zhì)量濃度葡萄糖注射實驗表明,在注射后30 min內(nèi),腸Pp GLUT1表達量升高,在30 min之后表達量逐漸降低,注射后第2小時表達量最低。不同濃度葡萄糖注射實驗表明,當葡萄糖質(zhì)量濃度為0.1~0.4 g·m L-1時,Pp GLUT1表達量逐漸上升;質(zhì)量濃度為0.4g·m L-1時,Pp GLUT1表達量最高;大于0.4 g·m L-1之后,表達量逐漸降低。上述結(jié)果表明,企鵝珍珠貝Pp GLUT1主要在腸和外套膜表達,并對葡萄糖注射有表達響應,可能在上述組織的葡萄糖轉(zhuǎn)運過程中發(fā)揮作用。
[Abstract]:The glucose transporter (GLUTs) is the key factor of energy metabolism, in order to explore the GLUT1 (glucose transporter type1) in Pteria penguin (Pteria Penguin) in the function, carry out the cloning and functional analysis of GLUT1 gene of Pteria penguin. A Pteria penguin GLUT1 gene cloned by RACE, and named Pp GLUT1. The gene C DNA has a total length of 2068 BP, encoding 522 amino acids, and has 2 transmembrane helical regions. The N ends and C ends are on the cytoplasmic side, the molecular weight is 57.2264 K D, and the theoretical isoelectric point is 5.17. Different quantitative results show that in the intestine and the mantle of the highest expression level, the expression of adductor muscle was significantly lower than other groups. 0.5 g. M L-1 high glucose injection experiment showed that the expression of Pp GLUT1 increased in 30 min after injection, and the expression level gradually decreased after 30 min, and the lowest expression was second hours after injection. Glucose injection experiments with different concentrations showed that when the concentration of glucose was 0.1~0.4 G. M L-1, the expression of Pp GLUT1 increased gradually. When the mass concentration was 0.4g? M L-1, the expression level of Pp GLUT1 was the highest, and the expression level decreased gradually when the concentration of glucose was greater than 0.4. The results show that Pp GLUT1 Pteria penguin is mainly expressed in the intestine and mantle, and expressed in response to glucose injection, glucose transporters may play a role in the process of the organization.
【作者單位】： 中國水產(chǎn)科學研究院南海水產(chǎn)研究所中國水產(chǎn)科學研究院南海水產(chǎn)種質(zhì)資源與健康養(yǎng)殖重點實驗室農(nóng)業(yè)部南海漁業(yè)資源開發(fā)利用重點實驗室南海生物資源開發(fā)與利用協(xié)同創(chuàng)新中心;上海海洋大學水產(chǎn)與生命學院;
【基金】：國家自然科學基金項目(31372525) 現(xiàn)代農(nóng)業(yè)(貝類)產(chǎn)業(yè)技術(shù)體系建設專項資金(CARS-48) 海南省重點科技計劃項目(ADXM20120032) 中國水產(chǎn)科學研究院基本科研業(yè)務費項目(2014A07XK02)
【分類號】：S917.4
【正文快照】： 葡萄糖是自然界生物的主要能量來源之一,維持生命正常代謝活動。葡萄糖的運輸需要葡萄糖轉(zhuǎn)運蛋白(glucose transporters,GLUTs)的參與。在NCBI中已經(jīng)登錄的GLUT基因亞型包括GLUT1、GLUT2、GLUT3、GLUT4、GLUT5、GLUT7、GLUT8和GLUT12,其中GLUT1是最早發(fā)現(xiàn)的葡萄糖轉(zhuǎn)運體,在生，

本文編號：1338489