本文選題：羥基磷灰石 + 等離子噴涂��； 參考：《新疆醫(yī)科大學》2015年碩士論文

【摘要】：目的:評價羥基磷灰石表面構建的壓電陶瓷涂層的生物相容性。方法:按照ISO/10993及GB/T16886系列標準,對羥基磷灰石表面構建的壓電陶瓷涂層進行檢測。1.溶血試驗:在稀釋抗凝兔血中分別加入羥基磷灰石表面構建的壓電陶瓷涂層浸提液、生理鹽水與蒸餾水,檢測溶血率;2.短期全身毒性試驗(經(jīng)口途徑):對大鼠分別灌胃給予羥基磷灰石表面構建的壓電陶瓷涂層浸提液與生理鹽水,觀察動物每周的體重變化,食物利用率;3.熱原試驗:自新西蘭兔耳緣靜脈分別注射羥基磷灰石表面構建的壓電陶瓷涂層浸提液與生理鹽水,觀察動物體溫變化。4.致敏試驗:試驗組以羥基磷灰石表面構建的壓電陶瓷涂層浸提液與弗氏完全佐劑為供試液,對照組以生理鹽水溶液與弗氏完全佐劑為供試液。用最大劑量法進行致敏試驗,觀察動物的皮膚情況。結果:1.羥基磷灰石表面構建的壓電陶瓷涂層浸提液的溶血率為0.86%;2.灌胃一周后,試驗組與對照組大鼠體重增長量差異無統(tǒng)計學意義(P0.05),食物利用率分別為16.5%,16.9%,灌胃兩周后,試驗組與對照組大鼠體重增長量差異無統(tǒng)計學意義(P0.05),食物利用率分別為16.71%,17.1%;3.注射羥基磷灰石表面構建的壓電陶瓷涂層浸提液和生理鹽水后,試驗組兔體溫升高0.30℃,試驗組兔體溫升高總和為0.60℃;4.去除貼敷物24,48,72小時后,試驗組與對照組豚鼠的皮膚評分均小于1。結論:羥基磷灰石表面構建的壓電陶瓷涂層無溶血作用、無毒性、不致熱、無致敏作用,具有良好的生物相容性。
[Abstract]:Objective: to evaluate the biocompatibility of piezoelectric ceramic coating on hydroxyapatite. Methods: according to ISO/10993 and GB/T16886 series standards, the piezoelectric ceramic coating on hydroxyapatite surface was tested. Hemolysis test: dilute anticoagulant rabbit blood by adding hydroxy apatite on the surface of piezoelectric ceramic coating extract, physiological saline and distilled water, to detect the hemolysis rate of 2. 5%. Short-term systemic toxicity test (via oral approach: rats were given hydroxy apatite surface piezoelectric ceramic coating extract and physiological saline respectively, to observe the weekly body weight changes, food utilization rate of 3. 5%. Pyrogen test: piezoelectric ceramic coating extract and physiological saline were injected into the auricular vein of New Zealand rabbit respectively. The changes of body temperature of animals were observed. Sensitization test: the piezoelectric ceramic coating extractant and Freund's complete adjuvant on hydroxyapatite were used as the test solution in the test group, and the physiological saline solution and Freund's complete adjuvant in the control group were used as the test solution. The maximum dose method was used to sensitize animals to observe the skin condition. The result is 1: 1. The hemolysis rate of the piezoelectric ceramic coating solution constructed on hydroxyapatite is 0.86% and 2% respectively. After a week of gavage, there was no significant difference in body weight growth between the experimental group and the control group (P 0.05), but the food utilization rate was 16. 5% and 16. 9% respectively. After two weeks of gavage, there was no significant difference in body weight growth between the experimental group and the control group (P 0. 05), and the food utilization rate was 16. 71% and 17. 1% respectively. The temperature of rabbits in the experimental group was increased by 0.30 鈩，

本文編號：1979171