控制蛋白質(zhì)在材料表面的吸附和脫附在生物材料或醫(yī)學(xué)等方面十分重要。在不同的情況下,對蛋白質(zhì)的吸附和脫附有不同的要求。例如,對于細(xì)胞和組織在材料表面的增殖以及蛋白質(zhì)的富集和分離需要蛋白質(zhì)的吸附,而在生物技術(shù)和生物醫(yī)學(xué)材料領(lǐng)域如生物傳感器、含血的材料如人工血管、人造心臟等則需要抑制蛋白質(zhì)的吸附。因此需要設(shè)計一種能夠根據(jù)需求調(diào)控蛋白質(zhì)在其上的吸附和脫附的表面,這一點(diǎn)可以通過同時具有刺激響應(yīng)性和抗蛋白質(zhì)吸附性能的表面實(shí)現(xiàn)。本論文發(fā)展了一種可以實(shí)現(xiàn)蛋白質(zhì)可控吸附的新方法:在材料表面接枝上抗蛋白吸附聚合物刷(聚(2-甲基-2-噁唑啉)(PMOXA))和刺激響應(yīng)性聚合物刷(聚(4-乙烯基吡啶)(P4VP))。論文分為兩個部分。(1)分別通過2-甲基-2-噁唑啉(MOXA)的陽離子開環(huán)聚合(CROP)和4-乙烯基吡啶(4VP)的可逆加成-斷裂鏈轉(zhuǎn)移(RAFT)聚合,和隨后與甲基丙烯酸縮水甘油酯的無規(guī)和嵌段共聚制備了聚(2-甲基-2-噁唑啉-r-甲基丙烯酸縮水甘油酯)(PMOXA-r-GMA)刷狀共聚物和聚(4-乙烯基吡啶)-b-聚甲基丙烯酸縮水甘油酯)(P4VP-b-PGMA)。最后,將PMOXA-r-...

【文章頁數(shù)】：116 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
Abstract
Chapter 1 Introduction and Background
    1.1 Polymer Brushes
    1.2 Binary Polymer Brushes
    1.3 Binary Polymer Brushes for Controlled Bio-adhesion
        1.3.1 Oppositely Charged Binary Polymer Brushes
        1.3.2 Hydrophobic Polymer Brushes
        1.3.3 Hydrophilic Polymer Brushes
    1.4 Poly(2-oxazoline)s (POXs)
        1.4.1 Poly(2-oxazoline) (POXs)
        1.4.2 CROP of Oxazolines
        1.4.3 Advantages of POXs
    1.5 Modification of Solid Substrate
        1.5.1 Pattering via "Grafting from" Method
        1.5.2 "Grafting to" Method
    1.6 Objective of This Thesis
    References
Chapter 2 Poly(2-methyl-2-ogazoline) and Poly(4-vinyl pyridine) Based Mixed Brusheswith Switchable Ability Toward Protein Adsorption
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Materials
        2.2.2 Polymers Synthesis
            2.2.2.1 Synthesis of PMOXA-r-GMA
            2.2.2.2 Synthesis of P4VP-b-PGMA
        2.2.3 Surface Modification
        2.2.4 Characterization
            2.2.4.1 Nuclear Magnetic Resonance (NMR)
            2.2.4.2 Fourier Transforms Infrared Spectroscopy (FTIR)
            2.2.4.3 X-ray Photoelectron Spectroscopy (XPS)
            2.2.4.4 Variable Angle Spectroscopic Ellipsometry (VASE)
            2.2.4.5 Atomic Force Microscopy (AFM)
        2.2.5 Switchable Properties
            2.2.5.1 Dry Thickness
            2.2.5.2 Water Contact Angle (WCA)
            2.2.5.3 FITC-BSA Adsorption Assay
            2.2.5.4 Quantification of Protein Adsorption
    2.3 Results and Discussions
        2.3.1 Synthesis of Polymers
        2.3.2 Characterization of Polymer Modified Surface
        2.3.3 Switchable Properties
            2.3.3.1 Thickness
            2.3.3.2 Hydrophilicity
            2.3.3.3 Protein Adsorption Behavior
    2.4 Conclusion
    References
Chapter 3 Reversible Adsorption of Protein on Poly(2-methyl-2-oxazoline) /Poly(4-vinylpyridine) Based Binary Polymer Brushes: Influence of Ionic Strength and P4VP ChainLength
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Materials
        3.2.2 Polymer Synthesis
            3.2.2.1 Synthesis of PMOXA-r-GMA
            3.2.2.2 Synthesis of P4VP-b-PGMA
        3.2.3 Surface Modification
        3.2.4 Characterizations
            3.2.4.1 Nuclear Magnetic Resonance (NMR)
            3.2.4.2 Zeta Potential Assessment
            3.2.4.3 X-ray Photoelectron Spectroscopy (XPS)
            3.2.4.4 Variable Angle Spectroscopic Ellipsometry (VASE)
            3.2.4.5 Atomic Force Microscopy (AFM)
        3.2.5 Switchable Properties
            3.2.5.1 Hydrophilicity
            3.2.5.2 Dry Thickness
            3.2.5.3 Qualitative Analysis of the Protein Adsorption
            3.2.5.4 QCM-D Experiments
    3.3 Results and Discussion
        3.3.1 Synthesis of Polymers
        3.3.2 Characterization of Polymer Modified Surfaces
        3.3.3 Switchable Properties
            3.3.3.1 Hydrophilicity of the Coating
            3.3.3.2 Thickness of the Coating
            3.3.3.3 Zeta Potential Measurements
        3.3.4 Protein Adsorption Studies
    3.4 Conclusion
    References
Chapter 4 Overall Conclusion and Future Work
Acknowledgements
List of Publications



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