在本論文中,我們設計了具有不同形狀、結構和表面性質的上轉換納米粒子,應用于構建生物傳感器和上轉換熒光成像,具體研究成果如下:1.合成了具有三維上轉換核-雙殼納米粒子（UCNDs）,包括一個過渡層（NaYF4:Yb,Er,Ca）包覆的活性核（NaYF4:Yb,Ca）和一個活性外殼（NaNdF4:Yb,Ca）。外枝晶殼中高濃度的Nd3+致敏劑增強了發(fā)光強度,而富含Yb3+的過渡層在外殼與內核之間起到了有效的能量遷移作用,防止了 Nd3+造成的不正常的猝滅效應。這些獨特的結構和成分優(yōu)勢使其在980 nm激發(fā)下的上轉換發(fā)光（UCL）分別比 NaYF4:Yb,Er,Ca@NaYF4:Yb,Ca 核殼 UCNPs 和NaYF4:Yb,Er,Ca 球形核 UCNPs 提高了5倍和 15倍。SiO2-COOH 涂層的 UCNDs（UCND@SiO2-COOH）作為高效的長期發(fā)光探針被成功地用于體外和體內生物成像,且無明顯毒性。UCND@SiO2-COOH的攝取和滯留幾乎在肝臟和脾臟中。本研究可能為制備具有生物醫(yī)學應用價值的三維UCNDs納米結構開辟道路。2.開發(fā)了一種簡便的可擴展方法,用于理性設計多維... 

【文章來源】：中國科學技術大學安徽省 211工程院校 985工程院校

【文章頁數(shù)】：109 頁

【學位級別】：博士

【文章目錄】：
摘要
Abstract
Chapter 1:Introduction
    1.1 Upconversion phenomenon and upconversion nanoparticles
        1.1.1 Upconversion phenomenon
        1.1.2 Upconversion Mechanisms
            1.1.2.1 Excited-state absorption (ESA)
            1.1.2.2 Energy Transfer Upconversion (ETU)
            1.1.2.3 Cooperative Sensitization Upconversion (CSU)
            1.1.2.4 Cooperative upconversion (CU)
            1.1.2.5 The Cross relaxation (CR)
            1.1.2.6 Photon Avalanche (PA)
            1.1.2.7 Energy Migration Upconversion (EMU)
            1.1.2.8 Energy cascaded upconversion (ECU)
        1.1.3 Synthetic methods of upconversion nanoparticles
            1.1.3.1 Hydro (Solvo) thermal Synthesis
            1.1.3.2 Thermal Decomposition Synthesis
        1.1.4 Surface functionalization of hydrophobic UCNPs
        1.1.5 The Bioanalytical and Biomedical Applications of UCNPs
        1.1.6 Core-shell, Core-double-shell upconversion Nanostructures
        1.1.7 Investigation of the multidimensional and multilayered upconversionNanoarchitectures
            1.1.7.1 Morphology control of the multidimensional and multilayeredupconversion Nanoarchitectures
        1.1.8 Absorption of UCNPs
        1.1.9 The novelty of the work
Chapter 2:Rational Synthesis of three-dimensional Core-double ShellUpconversion Nanodendrites with Ultrabright Luminescence for Bioimaging Application
    2.1 Abstract
    2.2 Introduction
    2.3 Experimental
        2.3.1 Chemicals and Materials
        2.3.2 Materials Characterization
        2.3.3 Synthesis of UCNDs
        2.3.4 Synthesis of UCND@SiO_2-COOH
        2.3.5 Cytotoxicity Study of UCND@SiO_2-COOH
        2.3.6 UCL Imaging of Cells
        2.3.7 In vivo UCL Imaging of UCND@SiO_2-COOH
        2.3.8 In Vivo Toxicology Analysis of UCND@SiO_2-COOH
    2.4 Results and discussion
    2.5 Conclusion
Chapter 3:Smart Design of Exquisite Multidimensional Multilayered Sand-clock-like Upconversion Nanostructures with Ultrabright Luminescenceas Efficient Luminescence Probes for Bioimaging Application
    3.1 Abstract
    3.2 Introduction
    3.3 Experimental section
        3.3.1 Materials
        3.3.2 Characterization
        3.3.3 Synthesis of UCNPs core
        3.3.4 Synthesis of UCNPs core-shell
        3.3.5 Synthesis of multidimensional UCCKs
        3.3.6 Synthesis of UCCK@SiO_2-COOH
        3.3.7 Measurement of Upconversion Luminescence Quantum Yields
        3.3.8 Cytotoxicity Study of UCCK@SiO_2-COOH
        3.3.9 In vitro experiments
        3.3.10 Surface modification of UCCK@SiO_2-COON with folic acid (FA)
        3.3.11 UCCK@SiO_2-FA specific targeting ability study
    3.4 Results and discussion
        3.4.1 Fabrication of the multidimensional UCCKs
        3.4.2 Growth mechanism of the multidimensional UCCKs
        3.4.3 Characterization of the multidimensional UCCKs
        3.4.4 Upconversion luminescence of the multidimensional UCCKs
        3.4.5 Cell imaging
    3.5 Conclusion
Chapter 4: Conclusion and Prospectives
    4.1 Conclusion
    4.2 Prospectives
References
Acknowledgements
Curriculum vitae
List of Publications



本文編號：3488979