【摘要】：目的:建立HPLC法同時測定不同產(chǎn)地假馬齒莧中5個皂苷(假馬齒莧皂苷I、苦艾素A3、假馬齒莧皂苷Ⅱ、假馬齒莧皂苷C異構體和假馬齒莧皂苷C)的含量。方法:假馬齒莧以50%乙醇水溶液提取;采用Agela Promsil C_(18)色譜柱(4.6 mm×250 mm,5μm),流動相為乙腈-0.2%磷酸水溶液(氫氧化鈉溶液調至p H 3.0)(32.5∶67.5),流速為1.0 m L·min~(-1),檢測波長為205 nm,柱溫為30℃。結果:上述5個皂苷的線性關系良好(r≥0.999 6),平均加樣回收率為98.6%~100.4%。不同生長環(huán)境的假馬齒莧中的5個成分各自含量有所差別,10批樣品中假馬齒莧皂苷I、苦艾素A3、假馬齒莧皂苷Ⅱ、假馬齒莧皂苷C異構體和假馬齒莧皂苷C的含量范圍分別為4.22~5.62、1.61~3.04、2.62~4.94、0.38~4.53和2.14~6.33 mg·g~(-1)。結論:該方法可用于假馬齒莧的質量控制。
[Abstract]:Objective: To establish an HPLC method for the simultaneous determination of the contents of 5 soap-1 (pseudo-amodentate soap-I, artemisinin A3, false-horse-tooth-type soap-II, pseudo-horse-tooth soap-C-isomer and pseudo-amodentate soap-C) in different producing areas. The method comprises the following steps of: extracting a false horse tooth powder with an aqueous solution of 50% ethanol; adopting an Agela Promega C _ (18) chromatographic column (4.6 mm to 250mm,5 & mu; m), wherein the mobile phase is acetonitrile-0.2% phosphoric acid aqueous solution (the sodium hydroxide solution is adjusted to pH 3.0) (32.5: 67.5), the flow rate is 1.0m L 路 min-(-1), and the detection wavelength is 205nm, The column temperature was 30 鈩，

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