【摘要】：采用液質(zhì)聯(lián)用法對生脈注射液中莽草酸成分進(jìn)行確認(rèn),建立高效液相色譜法測定生脈注射液中莽草酸含量。采用液質(zhì)聯(lián)用法測定目標(biāo)峰的分子離子峰m/z:173.08[M-H]-,與莽草酸對照品對比確認(rèn)目標(biāo)峰為莽草酸成分;色譜柱采用Synergi Hydro-RP 80A(250 mm×4.6 mm,4μm),以20 mmol/L的KH2PO4(H3PO4調(diào)p H至2.5)溶液為流動相,流量為0.6 m L/min,檢測波長214 nm;莽草酸在0.02~0.64μg范圍內(nèi)呈良好的線性關(guān)系,r=0.999 8,精密度、重復(fù)性試驗(yàn)RSD分別為0.15%和1.49%,平均加樣回收率為99.2%,RSD=1.56%。該研究首次確認(rèn)生脈注射液中含有莽草酸成分,且該成分來源于五味子;含量測定方法準(zhǔn)確、可靠,可作為生脈注射液中五味子成分質(zhì)量控制檢測方法。
[Abstract]:Shikimic acid in Shengmai injection was confirmed by liquid mass spectrometry, and the content of shikimic acid in Shengmai injection was determined by high performance liquid chromatography (HPLC). The molecular ion peak m/z:173.08 of the target peak was determined by liquid mass spectrometry, and compared with shikimic acid reference, the target peak was confirmed to be shikimic acid, the chromatographic column was Synergi Hydro-RP 80A (250 mm 脳 4.6 mm, 4 渭 m), the mobile phase was 20 mmol/L KH2PO4 (H3PO4 adjusted pH to 2.5) solution, the flow rate was 0.6 mL 路min, and the detection wavelength was 214 nm;. Shikimic acid showed a good linear relationship in the range of 0.02 ~ 0.64 渭 g, r 鈮，

本文編號：2519133