基于光強(qiáng)傳輸方程相位成像的寬場相干反斯托克斯拉曼散射顯微背景抑制
發(fā)布時(shí)間：2019-07-08 15:07
【摘要】：相干反斯托克斯拉曼散射(CARS)顯微能夠?qū)悠返奶厥饣瘜W(xué)組分進(jìn)行選擇性成像,無需熒光標(biāo)記,在生物醫(yī)學(xué)領(lǐng)域被廣泛應(yīng)用.然而,傳統(tǒng)的CARS圖像往往存在非共振背景信號(hào).本文將基于光強(qiáng)傳輸方程的單光束相位成像技術(shù)用于CARS顯微成像,來抑制CARS的非共振背景信號(hào).該方法通過記錄樣品在三個(gè)相鄰平面上的CARS圖像,然后利用光強(qiáng)傳輸方程獲取CARS光場的相位分布,最后利用共振CARS信號(hào)和非共振背景信號(hào)在相位上的差異,實(shí)現(xiàn)了對(duì)背景噪聲的抑制.該方法無需參考光,通過三次測量可完成CARS的背景噪聲抑制,具有良好的應(yīng)用前景.
[Abstract]:Coherent anti-Stoke Slaman scattering (CARS) microscope can selectively image the special chemical components of samples without fluorescence labeling and is widely used in biomedical field. However, there are often non-resonant background signals in traditional CARS images. In this paper, a single beam phase imaging technique based on light intensity propagation equation is applied to CARS microscopic imaging to suppress the non-resonant background signal of CARS. In this method, the CARS images of the samples on three adjacent planes are recorded, and then the phase distribution of the CARS light field is obtained by using the light intensity transmission equation. Finally, the background noise is suppressed by using the phase difference between the resonance CARS signal and the non-resonance background signal. This method does not need reference light, and the background noise suppression of CARS can be completed by three measurements, and it has a good application prospect.
【作者單位】： 西安電子科技大學(xué)物理與光電工程學(xué)院;中國科學(xué)院西安光學(xué)精密機(jī)械研究所瞬態(tài)光學(xué)與光子技術(shù)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號(hào):61605150,61475187,61575154,61377008) 中央高�；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金(批準(zhǔn)號(hào):JB160511,XJS16005,JBG160502) the “Thüringer Ministerium für Bildung,Wissenschaft und Kultur”(TMBWK,projects:B578-06001,14.90HWP,B714-07037) and the “Carl Zeiss Stiftung”and the Federal Ministry of Education and Research,Germany(FKZ:13N10508)資助的課題~~
【分類號(hào)】：O657.37
本文編號(hào)：2511675
[Abstract]:Coherent anti-Stoke Slaman scattering (CARS) microscope can selectively image the special chemical components of samples without fluorescence labeling and is widely used in biomedical field. However, there are often non-resonant background signals in traditional CARS images. In this paper, a single beam phase imaging technique based on light intensity propagation equation is applied to CARS microscopic imaging to suppress the non-resonant background signal of CARS. In this method, the CARS images of the samples on three adjacent planes are recorded, and then the phase distribution of the CARS light field is obtained by using the light intensity transmission equation. Finally, the background noise is suppressed by using the phase difference between the resonance CARS signal and the non-resonance background signal. This method does not need reference light, and the background noise suppression of CARS can be completed by three measurements, and it has a good application prospect.
【作者單位】： 西安電子科技大學(xué)物理與光電工程學(xué)院;中國科學(xué)院西安光學(xué)精密機(jī)械研究所瞬態(tài)光學(xué)與光子技術(shù)國家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號(hào):61605150,61475187,61575154,61377008) 中央高�；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金(批準(zhǔn)號(hào):JB160511,XJS16005,JBG160502) the “Thüringer Ministerium für Bildung,Wissenschaft und Kultur”(TMBWK,projects:B578-06001,14.90HWP,B714-07037) and the “Carl Zeiss Stiftung”and the Federal Ministry of Education and Research,Germany(FKZ:13N10508)資助的課題~~
【分類號(hào)】：O657.37
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