高價銀配合物的合成、表征及抗菌性能研究
發(fā)布時間：2019-06-12 15:08
【摘要】：銀系抗菌材料由于高效、無毒、耐藥性低等特點被廣泛應(yīng)用在日用品、紡織和醫(yī)藥等領(lǐng)域。其中,高價銀抗菌材料比單價銀或納米銀具有更強(qiáng)的抗菌能力、抗氯離子干擾能力、良好的光穩(wěn)定性等優(yōu)點,是一種非常具有潛力的抗菌材料,正被人們所關(guān)注。高價銀氧化物是目前應(yīng)用較多的高價銀抗菌材料,但由于其高的氧化能力,易與別的基底材料發(fā)生反應(yīng),尤其是Ag2O3,很不穩(wěn)定,難以保存。已報道的高價銀配合物抗菌材料也存在穩(wěn)定性較差等問題。基于上述的分析,本文合成了幾種高價銀配合物,并對大腸桿菌(E.coli)和金黃色葡萄球菌(S.aureus)的抗菌性能進(jìn)行了研究,目前還未見相關(guān)文獻(xiàn)報道。具體的內(nèi)容如下:(1)采用吡啶、2-氨基吡啶為配體,合成了較穩(wěn)定的吡啶雙氮二價銀配合物([Ag(py)2(N2)2](OH)2)及2-氨基吡啶二價銀配合物([Ag(2-Apy)3(OH)](HSO4)·H2O),采用元素分析、紅外光譜(FT-IR)及X-射線光電子能譜(XPS)對配合物進(jìn)行了表征。通過生長抑制曲線和最小抑菌濃度(MIC)對配合物的抗菌性能進(jìn)行研究,兩種配合物在0.5μg/mL的低濃度下可對E.coli和S.aureus產(chǎn)生明顯的抑制作用,對E.coli的MIC值均為5μg/mL,對S.aureus的MIC值均為20μg/mL。光穩(wěn)定測試表明兩種配合物均具有優(yōu)越的光穩(wěn)定性。(2)采用模板合成法合成了硫酸亞乙基二雙胍,進(jìn)一步合成了其三價銀配合物,采用氫核磁共振(1HNMR)、元素分析、FT-IR及XPS對配體及配合物進(jìn)行了表征。亞乙基二雙胍三價銀配合物在0.5μg/mL的濃度下對E.coli和S.aureus可產(chǎn)生抑制作用,而硫酸亞乙基二雙胍即使在800μg/mL對兩種細(xì)菌生長也沒有表現(xiàn)出抑制作用,三價銀配合物對兩種細(xì)菌的MIC分別為10μg/mL和40μg/mL,同樣具有很好的光穩(wěn)定性。對上述兩種二價銀配合物及三價銀配合物的抗菌機(jī)理進(jìn)行初步了探究,根據(jù)實驗結(jié)果,我們推測:高價銀配合物在抗菌的過程中,發(fā)生氧化還原反應(yīng),并產(chǎn)生自由基。(3)合成了水溶性陰離子卟啉四-(4-磺酸鈉苯基)卟啉(Na4TPPS)及水溶性陽離子卟啉四-(4-N,N,N-三甲氨基苯基)卟啉(TAPP),并進(jìn)一步合成了其二價、三價銀配合物。采用1HNMR、紫外光譜、元素分析、FT-IR及XPS對卟啉及配合物進(jìn)行了表征。采用抑菌圈實驗、MIC、最小殺菌濃度(MBC)檢測以上物質(zhì)對E.coli和S.aureus的光毒性及暗毒性。結(jié)果表明:陽離子卟啉及其高價銀配合物比陰離子卟啉及其配合物對兩種細(xì)菌具有更明顯的抗菌效果,這與細(xì)菌的表面結(jié)構(gòu)相關(guān)。三價銀卟啉比二價銀卟啉及自由卟啉具有更強(qiáng)的暗毒性,自由卟啉和三價銀卟啉具有更強(qiáng)的光毒性。紫外光譜表明三價銀卟啉在黑暗的條件下就能產(chǎn)生自由基等活性物質(zhì),且在光照下產(chǎn)量更大。
[Abstract]:Silver antibacterial materials are widely used in daily necessities, textiles, medicine and other fields because of their high efficiency, non-toxicity and low drug resistance. Among them, high-priced silver antibacterial materials have stronger antibacterial ability, chloride ion interference resistance, good photostability and other advantages than monovalent silver or nano-silver. It is a very potential antibacterial material, which is being paid more and more attention. High price silver oxide is a kind of high price silver antibacterial material which is widely used at present, but because of its high oxidation ability, it is easy to react with other substrate materials, especially Ag2O3, is very unstable and difficult to preserve. It has been reported that the antibacterial materials of high valence silver complexes also have some problems, such as poor stability. Based on the above analysis, several high valence silver complexes were synthesized and the antibacterial properties of Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) were studied. The main contents are as follows: (1) stable pyridine bivalent silver complexes ([Ag (py) 2 (N 2) 2] (OH) 2) and 2 aminopyridinium bivalent silver complexes ([Ag (2-Apy) 3 (OH)] (HSO4) H 2O) were synthesized by using pyridine and 2 aminopyridines as ligands. The complexes were characterized by elemental analysis, infrared spectroscopy (FT-IR) and X ray photoelectron spectroscopy (XPS). The antibacterial properties of the complexes were studied by growth inhibition curve and minimum inhibitory concentration (MIC). The two complexes had obvious inhibitory effects on E.coli and S.aureus at low concentration of 0.5 渭 g / mL, and the MIC values of 5 渭 g / mL, for E.coli were 20 渭 g / mL. for S.aureus. The photostability test showed that the two complexes had superior photostability. (2) Ethylene dibiguanidine sulfate was synthesized by template synthesis, and its trivalent silver complex was further synthesized. The ligands and complexes were characterized by hydrogen nuclear magnetic resonance (1HNMR), elemental analysis, FT-IR and XPS. Ethyl dibiguanidine trivalent silver complexes can inhibit E.coli and S.aureus at the concentration of 0.5 渭 g / mL, while ethyldibiguanidine sulfate has no inhibitory effect on the growth of the two bacteria even at 800 渭 g / mL. The MIC of trivalent silver complexes is 10 渭 g / mL and 40 渭 g / mL, respectively. The antibacterial mechanism of the above two silver complexes and trivalent silver complexes was preliminarily studied. according to the experimental results, we speculate that the high valence silver complexes redox and produce free radicals in the antibacterial process. (3) Water-soluble anion porphyrin tetra-(4-sulfophenyl) porphyrin (Na4TPPS) and water-soluble cationic porphyrin tetra-(4 鈮，
本文編號：2498091
[Abstract]:Silver antibacterial materials are widely used in daily necessities, textiles, medicine and other fields because of their high efficiency, non-toxicity and low drug resistance. Among them, high-priced silver antibacterial materials have stronger antibacterial ability, chloride ion interference resistance, good photostability and other advantages than monovalent silver or nano-silver. It is a very potential antibacterial material, which is being paid more and more attention. High price silver oxide is a kind of high price silver antibacterial material which is widely used at present, but because of its high oxidation ability, it is easy to react with other substrate materials, especially Ag2O3, is very unstable and difficult to preserve. It has been reported that the antibacterial materials of high valence silver complexes also have some problems, such as poor stability. Based on the above analysis, several high valence silver complexes were synthesized and the antibacterial properties of Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) were studied. The main contents are as follows: (1) stable pyridine bivalent silver complexes ([Ag (py) 2 (N 2) 2] (OH) 2) and 2 aminopyridinium bivalent silver complexes ([Ag (2-Apy) 3 (OH)] (HSO4) H 2O) were synthesized by using pyridine and 2 aminopyridines as ligands. The complexes were characterized by elemental analysis, infrared spectroscopy (FT-IR) and X ray photoelectron spectroscopy (XPS). The antibacterial properties of the complexes were studied by growth inhibition curve and minimum inhibitory concentration (MIC). The two complexes had obvious inhibitory effects on E.coli and S.aureus at low concentration of 0.5 渭 g / mL, and the MIC values of 5 渭 g / mL, for E.coli were 20 渭 g / mL. for S.aureus. The photostability test showed that the two complexes had superior photostability. (2) Ethylene dibiguanidine sulfate was synthesized by template synthesis, and its trivalent silver complex was further synthesized. The ligands and complexes were characterized by hydrogen nuclear magnetic resonance (1HNMR), elemental analysis, FT-IR and XPS. Ethyl dibiguanidine trivalent silver complexes can inhibit E.coli and S.aureus at the concentration of 0.5 渭 g / mL, while ethyldibiguanidine sulfate has no inhibitory effect on the growth of the two bacteria even at 800 渭 g / mL. The MIC of trivalent silver complexes is 10 渭 g / mL and 40 渭 g / mL, respectively. The antibacterial mechanism of the above two silver complexes and trivalent silver complexes was preliminarily studied. according to the experimental results, we speculate that the high valence silver complexes redox and produce free radicals in the antibacterial process. (3) Water-soluble anion porphyrin tetra-(4-sulfophenyl) porphyrin (Na4TPPS) and water-soluble cationic porphyrin tetra-(4 鈮，
本文編號：2498091
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