【摘要】：建立了快速測(cè)定禽蛋中氯霉素、氟苯尼考及氟苯尼考胺的超高效液相色譜-串聯(lián)質(zhì)譜分析方法。禽蛋用乙腈提取后,正己烷液液萃取除脂,濃縮后超高效液相色譜分離,以乙腈和10%甲醇水為流動(dòng)相進(jìn)行梯度洗脫,MRM反應(yīng)監(jiān)測(cè)模式檢測(cè)。結(jié)果表明在5~200μg/L的系列濃度范圍內(nèi)均呈良好線性關(guān)系,相關(guān)系數(shù)均大于0.999。氯霉素、氟苯尼考、氟苯尼考胺的檢測(cè)限為0.5μg/kg,定量限為2.0μg/kg,在2~20μg/kg添加濃度范圍內(nèi)平均回收率為60%~100%,精密度小于20%。該方法方便快捷、定性準(zhǔn)確,適于禽蛋中的氯霉素、氟苯尼考及氟苯尼考胺藥物殘留檢測(cè)。
[Abstract]:An ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) method for the rapid determination of chloramphenicol, florfenicol and florfenicolamine in poultry eggs was established. After extraction with acetonitrile, n-hexane liquid-liquid extraction and lipid removal, concentrated ultra-high performance liquid chromatography (HPLC), gradient eluting with acetonitrile and 10% methanol water as mobile phase, MRM reaction monitoring mode detection. The results show that there is a good linear relationship in the range of 5 ~ 200 渭 g / L, and the correlation coefficient is more than 0.999. The detection limit of chloramphenicol and flunphenicol was 0.5 渭 g / kg,. The quantitative limit was 2.0 渭 g / kg,. The average recovery was 60% 鈮，

本文編號(hào)：2498035