【摘要】：電化學(xué)傳感器因其分析速度快、成本低、操作簡(jiǎn)便及易于實(shí)現(xiàn)微型化等特點(diǎn),在分析化學(xué)、食品安全、環(huán)境監(jiān)測(cè)、臨床醫(yī)學(xué)和新藥篩選等領(lǐng)域有著重要的應(yīng)用價(jià)值。納米材料具有比表面積大、導(dǎo)電性好、優(yōu)良的催化特性和生物相容性,可以有效改善傳感器的分析性能,因此被廣泛應(yīng)用于構(gòu)建電化學(xué)傳感器的修飾材料。本論文基于三維有序多孔材料,分別制備了三種修飾電極,并將其應(yīng)用于藥物分子的電化學(xué)研究及定量檢測(cè)。主要研究結(jié)果如下:(1)采用模板法制備三維有序金摻雜納米TiO2多孔結(jié)構(gòu)的修飾電極(3DOM/ITO),并在該修飾電極表面固定抗菌素卡那霉素(Kana)的適配體(aptamer),從而構(gòu)建一種新型的適配體生物傳感器(Kana-aptamer/3DOM/ITO)。利用透射電鏡(TEM)、掃描電鏡(SEM)、循環(huán)伏安法(CV)、電化學(xué)阻抗譜(EIS)等方法對(duì)修飾電極的形貌和電化學(xué)性質(zhì)進(jìn)行表征。在優(yōu)化條件下,將該修飾電極應(yīng)用于牛奶中Kana殘留量的檢測(cè)。Kana的濃度與Fe(CN)63-/4-在適配體修飾電極上的氧化峰電流降低值與成正相關(guān),其測(cè)定線性范圍為8.6×10-7～1.7×10-4molL-1 檢出限為 7.0×10-8molL-1。(2)在3DOM/ITO電極表面通過(guò)電化學(xué)聚合制備木犀草素(Lu)印跡電極(MIP/3DOM/ITO)。采用SEM、CV、EIS及差分脈沖伏安法(DPV)等表征電極表面形貌及電化學(xué)性質(zhì)。討論支持電解質(zhì)pH、電聚合圈數(shù)、模板分子洗脫條件及富集時(shí)間等因素對(duì)印跡電極分析性能及Lu電流響應(yīng)的影響。在優(yōu)化實(shí)驗(yàn)條件下,Lu的氧化峰電流與其濃度在5×10-8～3×10-5molL-1范圍內(nèi)呈線性關(guān)系,檢測(cè)限為2.4×10-8 molL-1(S/N=3)。此外,將該印跡電極成功用于定量測(cè)定中成藥獨(dú)一味膠囊中Lu的含量。(3)通過(guò)水熱法制備納米級(jí)金屬-有機(jī)骨架材料(MOF) Nano-PCN-222,以Nano-PCN-222作為修飾材料構(gòu)建了一種新的MOF電化學(xué)傳感器,并用于氨茶堿(AP)的定量檢測(cè)。AP在該修飾電極上有一較強(qiáng)氧化峰,且電流強(qiáng)度明顯高于玻碳電極,表明Nano-PCN-222能有效地促進(jìn)氨茶堿在電極表面的電化學(xué)反應(yīng),從而提高氨茶堿在修飾電極上的電化學(xué)響應(yīng)及檢測(cè)靈敏度。采用SEM、X-射線衍射(XRD)等手段對(duì)制備的MOF材料進(jìn)行了結(jié)構(gòu)和形貌表征。討論了實(shí)驗(yàn)條件,如:Nano-PCN-222固載量、支持電解質(zhì)、溶液pH、模板分子富集時(shí)間等對(duì)氨茶堿測(cè)定的影響。在優(yōu)化條件下,檢測(cè)氨茶堿的濃度線性范圍為5.0×10-7～1.5×10-4molL-1,檢出限為6.0×10-8mol L-1 (S/N=3),并實(shí)現(xiàn)了對(duì)氨茶堿注射液和臨床血樣中氨茶堿的定量檢測(cè)。
[Abstract]:Electrochemical sensors have important application value in analytical chemistry, food safety, environmental monitoring, clinical medicine and new drug screening because of their fast analysis speed, low cost, simple operation and easy miniaturization. Nanomaterials have large specific surface area, good conductivity, excellent catalytic properties and biocompatibility, and can effectively improve the analytical performance of the sensor, so they are widely used in the construction of electrochemical sensor modification materials. In this paper, three kinds of modified electrodes were prepared based on three-dimensional ordered porous materials and applied to the electrochemical study and quantitative detection of drug molecules. The main results are as follows: (1) the modified electrode (3DOM/ITO) with three-dimensional ordered gold-doped nano-TiO2 porous structure was prepared by template method, and the aptamer (aptamer), of antibiotic kanamycin (Kana) was fixed on the surface of the modified electrode. A new type of adaptive biosensor (Kana-aptamer/3DOM/ITO) was constructed. The morphology and electrochemical properties of the modified electrode were characterized by transmission electron microscope (TEM),) scanning electron microscope (SEM),) cyclic Voltammetric method (CV),) electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the modified electrode was applied to the determination of Kana residues in milk. The concentration of Kana was positively correlated with the decrease of oxidation peak current of Fe (CN) 63 鈮，

本文編號(hào)：2487303