【摘要】：建立了鵝組織(肌肉、肝臟、腎臟以及皮膚和脂肪)中哌嗪殘留的氣相色譜-串聯(lián)質(zhì)譜檢測(cè)法。鵝組織經(jīng)加速溶劑萃取(ASE350),乙腈提取,過Strata-X-C固相萃取小柱凈化后的樣品在乙酸酐和三乙胺條件下衍生。衍生產(chǎn)物經(jīng)氣相色譜-串聯(lián)質(zhì)譜儀(GC-MS/MS)檢測(cè),色譜柱為TG-5MS Amine(30 m×0.25 mm i.d.,0.25μm),載氣為氦氣,進(jìn)樣口和檢測(cè)器溫度均為280℃。采用EI模式,SCAN和SIM掃描定性,Auto SRM結(jié)合外標(biāo)法定量。哌嗪添加量在5.1~2.5×10~3μg/kg范圍內(nèi),線性關(guān)系良好,相關(guān)系數(shù)為0.9995。在定量限(LOQ:5.1,5.2,5.2,5.2)~2.0×10~3μg/kg的加標(biāo)水平上,鵝組織中哌嗪的平均回收率為85.2%~95.5%,相對(duì)標(biāo)準(zhǔn)偏差(RSD)為1.6%~4.7%,日內(nèi)RSD為1.2%~5.3%,日間RSD為2.0%~7.7%。檢測(cè)限(LOD)和定量限(LOQ)分別在1.4~1.6μg/kg(S/N≥3)和5.1~5.2μg/kg(S/N≥10)之間。方法適用于鵝組織中哌嗪殘留的檢測(cè)。
[Abstract]:A gas chromatography-tandem mass spectrometry (GC-MS) method for the determination of piperazin residues in goose tissues (muscle, liver, kidney, skin and fat) was established. The samples purified by accelerated solvent extraction (ASE350), acetonitrile extraction and Strata-X-C solid phase extraction column were derived from goose tissue under the conditions of acetic anhydride and triethylamine. The derived products were detected by gas chromatography-tandem mass spectrometry (GC-MS/MS). The chromatographic column was TG-5MS Amine (30 m 脳 0.25 mm I.D., 0.25 渭 m), the carrier gas was Helium, and the injection port and detector temperature were 280 鈩，

本文編號(hào)：2487127