【摘要】：目的建立重樓藥材的HPLC指紋圖譜,并測(cè)定4種重樓皂苷的含量。方法采用HPLC法,Thermo BDS Hypersil C18(4.6mm×250mm,5μm)色譜柱,以乙腈-水為流動(dòng)相梯度洗脫,流速為0.8 ml·min-1,檢測(cè)波長(zhǎng)為203 nm,柱溫為30℃。對(duì)10批重樓藥材進(jìn)行分析,建立重樓藥材的指紋圖譜,同時(shí)測(cè)定了重樓皂苷Ⅰ ,Ⅱ , Ⅵ ,Ⅶ 4個(gè)成分的含量。結(jié)果初步建立了重樓HPLC特征指紋圖譜共有模式,標(biāo)定了13個(gè)共有峰。通過(guò)對(duì)照品歸屬法,確定了重樓皂苷I、重樓皂苷II、重樓皂苷VI和重樓皂苷VII共4個(gè)化合物,10批重樓相似度在0.851~0.970之間。4種重樓皂苷的含量測(cè)定線性良好(r0.9995,n=5),重樓皂苷I,II,VI,VII的平均回收率為99.41%,99.51%,94.77%,96.57%,RSD分別為1.20%,1.27%,2.55%,1.32%。結(jié)論該方法簡(jiǎn)便快捷,重復(fù)性好,可以用于重樓藥材的鑒定與評(píng)價(jià),為控制重樓藥材的質(zhì)量提供可靠的科學(xué)依據(jù)。
[Abstract]:Objective to establish the HPLC fingerprint of Tripterygium wilfordii and determine the contents of four kinds of saponins. Methods, Thermo BDS Hypersil C18 (4.6mm 脳 250mm, 5 渭 m) column was used by HPLC. The mobile phase was eluted with acetonitrile-water as mobile phase. The flow rate was 0.8 ml 路min-1 and the detection wavelength was 203 nm, column temperature at 30 鈩，

本文編號(hào)：2481369