【摘要】：目的:利用UPLC-QQQ-MS建立復(fù)雜中藥基質(zhì)中馬兜鈴酸Ⅰ的定性定量測定方法。方法:以馬兜鈴科中藥材及其易于混淆的中藥材為研究對象,色譜分離使用Agilent ZORBAX XDB-C18色譜柱(2.1 mm×50 mm,3.5μm),流動相0.2%甲酸水溶液-甲醇梯度洗脫,流速0.2 m L·min~(-1),柱溫35℃,采用電噴霧離子源(ESI源),正離子模式下,多反應(yīng)監(jiān)測模式(MRM)檢測,以吲哚美辛為內(nèi)標(biāo)計算濃度。結(jié)果:馬兜鈴酸Ⅰ質(zhì)量濃度在21.65~1 732μg·L~(-1)線性關(guān)系良好(r=0.997 2),加樣回收率為82.87%~89.22%,平均加樣回收率86.07%(n=6),RSD 2.5%,精密度及方法重復(fù)性均良好。基于這個方法筆者將從全國各地收集來的中藥材進行定向馬兜鈴酸Ⅰ含量的檢測,結(jié)果精準(zhǔn)可信。結(jié)論:該方法簡便,快速,靈敏度高,專屬性強,可以用于含有或者可能含有馬兜鈴酸Ⅰ成分中藥材的檢測。結(jié)果表明,不同種類藥材中馬兜鈴酸Ⅰ的含量差異明顯,尤以朱砂蓮中馬兜鈴酸Ⅰ含量最高。同時,該檢測結(jié)果也可為馬兜鈴科中藥材中馬兜鈴酸Ⅰ的限量及其合理使用提供實驗基礎(chǔ)和參考依據(jù)。
[Abstract]:Objective: to establish a qualitative and quantitative method for the determination of aristolochic acid I in complex traditional Chinese medicine matrix by UPLC-QQQ-MS. Methods: Aristolochaceae and its confusing Chinese medicinal materials were used as research objects. Agilent ZORBAX XDB-C18 column (2.1 mm 脳 50 mm, 3.5 渭 m) was used for chromatographic separation. The mobile phase was 0.2% formic acid aqueous solution-methanol gradient eluting. The flow rate was 0.2mL 路min ~ (- 1), the column temperature was 35 鈩，

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