【摘要】：利用高效毛細管區(qū)帶電泳法,對3個產地沙棘果粉中的蘆丁、山柰酚、異鼠李素、槲皮素4種活性物質進行同時分離測定�？疾觳煌娪緱l件對分離效率的影響,得出最佳電泳條件:緩沖溶液20 mmol/L Na_2B_4O_7-H_3BO_3(質量濃度為1.5 mg/mL的β-環(huán)糊精),pH 9.55,檢測波長370 nm,分離電壓25 kV,進樣時間5 s。在該條件下,蘆丁、山柰酚、異鼠李素、槲皮素4種活性物質在11 min內得到分離,線性范圍分別為0.01~0.51、0.05~0.93、0.02~0.65、0.03~0.81 mg/mL,相關系數(shù)在0.997 1~0.999 1之間,檢出限分別為5.05×10~(-5)、2.10×10~(-5)、3.75×10~(-5)、1.31×10~(-5) mg/mL(RSN=3),各物質線性關系良好。日內及日間精密度范圍為0.08%~4.72%,平均回收率在95.51%~104.66%之間,相對標準偏差不大于3.92%(n=3)。在優(yōu)化條件下,對4種活性物質標準品的混合物進行測定,并用相同方法對3個產地沙棘果粉中活性物質的提取液進行檢測。結果表明:不同產地沙棘中,4種被檢測化合物的含量各不相同。沙棘中被測4種化合物總量最高的產自新疆烏魯木齊,最低的產自甘肅武威,而槲皮素含量以產自山西呂梁的最高,山柰酚和異鼠李素以產自新疆烏魯木齊最高。本方法快速簡便、準確可靠,可用于沙棘果粉中多種活性物質的同時檢測。
[Abstract]:Four active substances (rutin, kaempferol, isorhamnetin and quercetin) in Hippophae rhamnoides powder from three producing areas were separated and determined by high performance capillary zone electrophoresis (HPCE). The effects of different electrophoretic conditions on the separation efficiency were investigated, and the optimum electrophoretic conditions were obtained as follows: buffer solution 20 mmol/L Na_2B_4O_7-H_3BO_3 (mass concentration 1.5 mg/mL 尾-cyclodextrin), pH 9.55, p-cyclodextrin), pH 9.55, mass concentration 1.5 mg/mL), Detection wavelength 370 nm, separation voltage 25 kV, injection time 5 s. Under these conditions, four active substances, rutin, kaempferol, isorhamnetin and quercetin, were separated in 11 min. The linear ranges were 0.01 ~ 0.51, 0.05 ~ 0.93, 0.02 ~ 0.65, 0.03 ~ 0.81 mg/mL, respectively. The correlation coefficients were 0.997 脳 10 ~ (- 5) mg/mL (RSN=3), and the detection limits were 5.05 脳 10 ~ (- 5), 2.10 脳 10 ~ (- 5), 3.75 脳 10 ~ (- 5) and 1.31 脳 10 ~ (- 5) respectively. The intra-day and inter-day precision ranges are 0.08% and 4.72% respectively. The average recovery is between 95.51% and 104.66%. The relative standard deviation is less than 3.92% (n = 3). Under the optimized conditions, the mixture of four kinds of active substances was determined, and the extract of active substances from three producing areas of seabuckthorn fruit powder was detected by the same method. The results showed that the contents of four compounds in Hippophae rhamnoides from different producing areas were different. The highest total amount of four compounds in Hippophae rhamnoides was from Urumqi, Xinjiang, and the lowest was from Wuwei, Gansu Province. The quercetin content was the highest in Lv Liang, Shanxi, and the highest in kaempferol and isorhamnetin in Xinjiang Urumqi. The method is rapid, simple, accurate and reliable, and can be used for simultaneous determination of various active substances in seabuckthorn fruit powder.
【作者單位】： 山西師范大學化學與材料科學學院;山西大同大學化學與環(huán)境工程學院;
【基金】：國家自然科學基金面上項目(21375083)
【分類號】：O658.9;TS278

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1 趙思俊;趙曉U，

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