【摘要】：目的建立右歸飲的HPLC指紋圖譜,并同時(shí)測(cè)定9種成分(京尼平苷酸、莫諾苷、綠原酸、梔子苷、馬錢(qián)苷、松脂醇二葡萄糖苷、甘草苷、蘆丁和甘草酸)的量,為控制右歸飲的質(zhì)量提供依據(jù)。方法采用Pursuit XRs 5 C18色譜柱(250mm×4.6 mm,5μm)進(jìn)行分離,流動(dòng)相為乙腈-0.1%磷酸水溶液,梯度洗脫,體積流量1.0 m L/min,檢測(cè)波長(zhǎng)230 nm,柱溫30℃。建立10批右歸飲樣品的HPLC指紋圖譜,并進(jìn)行相似度評(píng)價(jià);通過(guò)與各組方藥味及對(duì)照品的保留時(shí)間和紫外光譜圖比對(duì),對(duì)共有峰進(jìn)行歸屬和指認(rèn),并測(cè)定指認(rèn)出的9個(gè)成分的量。結(jié)果 10批樣品指紋圖譜與對(duì)照指紋圖譜的相似度均大于0.904;共標(biāo)定30個(gè)共有峰,其中8個(gè)來(lái)自杜仲,8個(gè)來(lái)自甘草,6個(gè)來(lái)自山茱萸,2個(gè)來(lái)自附子,2個(gè)來(lái)自熟地黃,1個(gè)來(lái)自肉桂,山藥及枸杞對(duì)共有峰貢獻(xiàn)不明顯,其中3個(gè)共有峰不能明確其來(lái)源;通過(guò)對(duì)共有峰進(jìn)行指認(rèn),發(fā)現(xiàn)7號(hào)峰為京尼平苷酸、9號(hào)峰為莫諾苷、11號(hào)峰為綠原酸、12號(hào)峰為梔子苷、13號(hào)峰為馬錢(qián)苷、14號(hào)峰為松脂醇二葡萄糖苷、18號(hào)峰為甘草苷、21號(hào)峰為蘆丁及30號(hào)峰為甘草酸,并對(duì)這9種成分進(jìn)行了定量測(cè)定,其定量測(cè)定結(jié)果分別為87.6~119.1μg/g、323.6~365.6μg/g、108.3~124.1μg/g、79.5~85.0μg/g、171.7~188.0μg/g、163.0~238.3μg/g、64.5~53.3μg/g、159.8~168.5μg/g、72.8~83.6μg/g。結(jié)論所建立的右歸飲HPLC指紋圖譜及定量測(cè)定方法穩(wěn)定性、重復(fù)性好,可為右歸飲質(zhì)量控制和評(píng)價(jià)提供參考。
[Abstract]:Objective to establish a HPLC fingerprint of Yougui Yin, and to determine the contents of nine constituents (Geniposide, Monoside, chlorogenic Acid, Gardeniposide, Martinoside, Rosin diglucoside, Glycyrrhizin, rutin and glycyrrhizic acid), at the same time. It provides the basis for controlling the quality of Youguiyin. Methods Pursuit XRs-5 C18 column (250mm 脳 4.6 mm, 5 渭 m) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution, gradient elution, volume flow rate 1.0ml / min, detection wavelength 230nm, column temperature 30 鈩，

本文編號(hào)：2455206