【摘要】：目的制備桂枝飲片標(biāo)準(zhǔn)湯劑,并進(jìn)行質(zhì)量標(biāo)準(zhǔn)研究。方法依照標(biāo)準(zhǔn)湯劑的制備要求,制備15批桂枝飲片標(biāo)準(zhǔn)湯劑,以肉桂酸作為定量檢測(cè)指標(biāo),計(jì)算轉(zhuǎn)移率與出膏率,并建立其HPLC指紋圖譜分析方法。結(jié)果通過對(duì)15批桂枝標(biāo)準(zhǔn)湯劑進(jìn)行測(cè)定,肉桂酸轉(zhuǎn)移率為56.93%~94.06%,出膏率為3.30%~9.40%;并用中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)(2012A)軟件進(jìn)行指紋圖譜分析,標(biāo)定了9個(gè)共有峰,確認(rèn)4個(gè),分別為原茶兒酸(1號(hào)峰)、香豆素(4號(hào)峰)、肉桂酸(6號(hào)峰)、桂皮醛(7號(hào)峰)。對(duì)15批桂枝飲片標(biāo)準(zhǔn)湯劑分別進(jìn)行了相似度評(píng)價(jià),其相似度均高于0.95。結(jié)論建立了桂枝飲片標(biāo)準(zhǔn)湯劑指紋圖譜,該方法精密度、穩(wěn)定性和重復(fù)性良好,具有一定的鑒別意義,可為桂枝配方顆粒的質(zhì)量控制提供參考。
[Abstract]:Objective to prepare and study the quality standard of Guizhi decoction. Methods according to the requirement of preparation of standard decoction, 15 batches of standard decoction of Guizhi decoction were prepared. The transfer rate and extract rate were calculated with cinnamic acid as the quantitative detection index, and the HPLC fingerprint analysis method was established. Results the results showed that the transfer rate of cinnamic acid was 56.93% and 94.06%, and the ointment rate was 3.30% 9.40%. The chromatographic fingerprint similarity evaluation system (2012A) was used for fingerprint analysis. Nine common peaks were calibrated, and 4 peaks were identified as crude tea acid (peak 1), coumarin (peak 4) and cinnamic acid (peak 6). Cinnamaldehyde (peak 7). The similarity of 15 batches of standard decoction of Cinnamomum Cinnamomi was evaluated, and the similarity was higher than 0.95. Conclusion the fingerprint of Guizhi decoction was established. The method has good precision, stability and reproducibility, and can be used as a reference for the quality control of Guizhi prescription granules.
【作者單位】： 廣東藥科大學(xué)國(guó)家中醫(yī)藥管理局中藥數(shù)字化質(zhì)量評(píng)價(jià)技術(shù)重點(diǎn)研究室廣東高校中藥質(zhì)量工程技術(shù)研究中心;康美(北京)藥物研究院有限公司;廣東藥科大學(xué)中藥學(xué)院;
【基金】：廣東省戰(zhàn)略性新興產(chǎn)業(yè)區(qū)域集聚發(fā)展試點(diǎn)項(xiàng)目——中藥經(jīng)典配方顆粒重點(diǎn)試驗(yàn)平臺(tái)及中藥材全生命周期追溯信息服務(wù)平臺(tái)建設(shè)項(xiàng)目(2014793)
【分類號(hào)】：R286.0;O657.7

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