發(fā)布時間：2019-01-06 13:20

【摘要】：目的:通過對廣東傳統(tǒng)炮制醋馬錢子的研究,建立醋馬錢子的HPLC指紋圖譜,并探討炮制前、中、后指紋圖譜的變化。方法:采用HPLC法,色譜柱:Synergi 4u Polar-RP 80A色譜柱(250 mm×4.6 mm,5μm);流動相:A為水-乙酸-三乙胺(100∶0.2∶0.2),B為乙腈(梯度洗脫);流速:0.8 mL/min;檢測波長:254 nm;柱溫:25℃;進樣量:6μL。結(jié)果:醋馬錢子炮制品及其生品各相似度均大于0.97,相對保留時間的重現(xiàn)性好,炮制品RSD≤0.10%,生品RSD≤0.18%;炮制品和生品共有峰均達16個。結(jié)論:建立了醋馬錢子的HPLC指紋圖譜,并比較了醋馬錢子與其生品和中間品之間指紋圖譜的差異,研究結(jié)果對醋馬錢子的炮制機理提供了參考依據(jù)。
[Abstract]:Objective: to establish the HPLC fingerprint of semen strychni vinegar by traditional processing in Guangdong province, and to explore the change of fingerprint before, during and after processing. Methods: HPLC method was used. The chromatographic column was Synergi 4u Polar-RP 80A column (250 mm 脳 4.6 mm,5 渭 m); mobile phase: a was water-acetic acid-triethylamine (100: 0.2: 0.2), B) acetonitrile (gradient elution); Flow rate: 0. 8 mL/min; detection wavelength: 254 nm; column temperature: 25 鈩，

本文編號：2402839