【摘要】：目的:建立紅外光譜快速鑒別羌活和寬葉羌活兩種基原植物藥材的方法。方法:收集了8份羌活和4份寬葉羌活根莖和根藥材,用傅里葉變換紅外光譜技術測定4 000~400 cm~(-1)范圍內的一維紅外光譜和二維相關紅外光譜,計算二階導數光譜,定量測定峰的強度,并進行光譜解析、主成分分析。結果:羌活與寬葉羌活藥材一維紅外光譜的特征峰不同。羌活在1 739(吸光度為0.39)、1 428(0.46)、1 076(0.91)、863(0.03)、764(0.08)cm~(-1)處有明顯吸收峰,而寬葉羌活在此處的峰不明顯(強度小于閾值0.01);寬葉羌活在1 719(0.34)、1 607(0.50)、1 444(0.41)、823(0.06)、775(0.07)cm~(-1)處的吸收峰明顯,而羌活在此處的峰不明顯。在模式識別-主成分分析聚類圖中,羌活與寬葉羌活分布于不同區(qū)域。二階導數譜中兩者主要共有峰的強度明顯不同,羌活在1 747、1 468、1 159、1 078和988 cm~(-1)的峰強度明顯高于寬葉羌活。而寬葉羌活在1 627、1 605、1 568、1 512和1 269 cm~(-1)峰強度明顯高于羌活。二維相關紅外光譜中兩者自動峰的數量、位置及其相互關系不同。羌活樣品在850~1 500 cm~(-1)范圍內有13個自動峰,而寬葉羌活為9個自動峰。結論:根據一維、二階導數和二維相關紅外光譜,羌活與寬葉羌活兩種植物的藥材可快速鑒別。峰強度定量比較、主成分分析、聚類分析增加了鑒別的客觀性和準確性。
[Abstract]:Objective: to establish a method for rapid identification of Radix Notopterygii and Radix Notopterygii. Methods: eight samples of Radix Notopterygium and four samples of Radix Notopterygii rhizome and root were collected. The 1D and 2D IR spectra in the range of 400 cm~ (-1) were determined by Fourier transform infrared spectroscopy (FTIR), and the second derivative spectra were calculated. The intensity of the peak was determined quantitatively, and the spectral analysis and principal component analysis were carried out. Results: the characteristic peaks of one-dimensional infrared spectrum of Radix Notopterygii and Radix Notopterygii were different. There are obvious absorption peaks at 1 739 (absorbance 0. 39), 1 428 (0. 46), 1 076 (0. 91), 863 (0. 03), 764 (0. 08) cm~ (-1). But the peak of Notopterygium gibbergii was not obvious here (the intensity was less than the threshold value 0.01). The absorption peaks at 1 719 (0. 34), 1 607 (0. 50), 1 444 (0. 41), 823 (0. 06), 775 (0. 07) cm~ (-1) were obvious, but the peaks of Notopterygium Notopterygii were not obvious. In the pattern recognition-principal component analysis (PCA) cluster map, Notopterygium gibbergii and Notopterygium gibbergii are distributed in different regions. The intensity of the main common peaks in the second derivative spectrum was obviously different. The peak intensity of Notopterygium chinensis at 1 468 9 cm~ (-1 078) and 988 cm~ (-1) was significantly higher than that of Notopterygium gibbergii. However, the peak intensities of 1 627, 1 605 and 1 269 cm~ (-1) peaks of Notopterygium gibbergii were significantly higher than those of Notopterygium gibbergii. The number, position and relationship of the automatic peaks in two dimensional correlation infrared spectroscopy are different. In the range of 850 ~ 1 500 cm~ (-1), there are 13 automatic peaks in the sample of Notopterygium, and 9 peaks in the range of 1 500 cm~ (-1). Conclusion: according to one dimensional, second derivative and two dimensional correlation infrared spectroscopy, two kinds of medicinal herbs of Notopterygium and Notopterygium chinensis can be quickly identified. Quantitative comparison of peak intensity, principal component analysis and cluster analysis increased the objectivity and accuracy of identification.
【作者單位】： 成都中醫(yī)藥大學藥學院/中藥材標準化教育部重點實驗室;四川省食品藥品檢驗檢測院;四川省中醫(yī)藥科學院;珀金埃爾默企業(yè)管理(上海)有限公司;
【基金】：國家基礎科學人才培養(yǎng)基金項目(J1310034-02) 四川省科技支撐計劃項目(2014SZ0071-4) 成都中醫(yī)藥大學大學生科研實踐創(chuàng)新課題(ky2016-016)
【分類號】：O657.33;R284.1
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本文編號：2388992