發(fā)布時間：2018-11-20 05:16

【摘要】：基于夾心法免疫層析試條檢測原理,結(jié)合對流擴散方程和流體動力學方程,建立了夾心法免疫層析試條動態(tài)反應過程的數(shù)學模型,并通過COMSOL軟件對試條動態(tài)反應過程進行仿真。分別探究了目標待測物A濃度在0~20 mol/L,標記物P濃度在1×10!2~1×103mol/L以及硝酸纖維素膜的孔隙率在0~1范圍內(nèi)變化時,檢測線上夾心復合物濃度關(guān)于位置和時間的濃度變化情況,并分析了各物質(zhì)初始濃度以及試條結(jié)構(gòu)對于檢測結(jié)果和檢測時間的影響。結(jié)果表明,在一定濃度范圍內(nèi),目標待測物A以及標記物P濃度的增加將提高試條的定量檢測性能,而孔隙率通過影響混合液流速和混合液中各物質(zhì)反應接觸情況來影響檢測結(jié)果。
[Abstract]:Based on the principle of sandwich immunochromatographic strip detection, combined with convection diffusion equation and hydrodynamic equation, the mathematical model of the dynamic reaction process of sandwich immunochromatographic strip was established, and the dynamic reaction process of the strip was simulated by COMSOL software. When the concentration of target substance A is 0 ~ 20 mol/L, the concentration of P is 1 脳 10 ~ 2 ~ (-1) 103mol/L, and the porosity of nitrocellulose membrane is in the range of 0 ~ (-1). The change of the concentration of sandwich complex on the detection line about position and time was analyzed. The influence of the initial concentration of each substance and the structure of the sample strip on the detection results and the detection time were analyzed. The results showed that in a certain concentration range, the increase of the concentration of target substance A and marker P would improve the quantitative detection performance of the test strip. The porosity affects the detection results by influencing the flow rate of the mixture and the reaction contact of each substance in the mixture.
【作者單位】： 廈門大學儀器與電氣系;福州大學福建省醫(yī)療器械和醫(yī)藥技術(shù)重點實驗室;中國科學院深圳先進技術(shù)研究院;
【基金】：國家科技部國際合作項目(No.2009DFA32050) 國家自然科學基金(No.61403319) 福建省自然科學基金(No.2015J05131) 福建省生態(tài)產(chǎn)業(yè)綠色技術(shù)重點實驗室開放課題資助項目~~
【分類號】：O652

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