【摘要】：目的建立酸棗仁Ziziphi spinosae Semen黃酮HPLC指紋圖譜,并測定其中2種成分的含有量。方法以斯皮諾素為參照峰,建立10批樣品的HPLC指紋圖譜。酸棗仁黃酮甲醇提取物的分析采用Agilent TC-C_(18)色譜柱(4.6 mm×250 mm,5μm);流動相乙腈-0.1%醋酸,梯度洗脫;體積流量1 m L/min;柱溫30℃;檢測波長280 nm。結(jié)果 HPLC指紋圖譜中有10個共有峰,并鑒定出其中8個(維采寧-Ⅱ、牡荊素葡萄糖苷、異斯皮諾素、斯皮諾素、6'''-pyridyloylspinosin、6'''-對羥基苯甲酰斯皮諾素、6'''-阿魏酰斯皮諾素、6'''-對香豆酰斯皮諾素)。斯皮諾素和6'''-阿魏酰斯皮諾素分別在15.00~40.00μg和5.00~14.00μg范圍內(nèi)線性關(guān)系良好,平均加樣回收率(RSD)分別為100.5%(1.6%)和100.4%(1.6%)。結(jié)論該方法準確、簡單、可靠,可用于酸棗仁黃酮的質(zhì)量控制。
[Abstract]:Objective to establish the HPLC fingerprint of Ziziphi spinosae Semen flavonoids from jujube kernel and determine the content of two components. Methods the HPLC fingerprint of 10 batches of samples was established with spinosin as the reference peak. Analysis of flavonoid methanol extract from Ziziphus jujube seed by Agilent TC-C_ (18) column (4.6 mm 脳 250 mm,5 渭 m);) mobile phase acetonitrile-0.1% acetic acid, gradient elution; volume flow rate 1 mL / min; column temperature 30 鈩，

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