【摘要】：目的:建立同時(shí)測(cè)定風(fēng)濕關(guān)節(jié)炎片中鹽酸麻黃堿、鹽酸偽麻黃堿、馬錢子堿、士的寧含量的方法。方法:采用高效液相色譜法。色譜柱為XTerra-C_(18),流動(dòng)相為乙腈-0.01 mol/L庚烷磺酸鈉與0.01 mol/L磷酸二氫鉀的等量混合溶液(用磷酸調(diào)p H至2.8)(梯度洗脫),流速為1.0 ml/min,檢測(cè)波長(zhǎng)為206 nm(鹽酸麻黃堿和鹽酸偽麻黃堿)、260 nm(馬錢子堿和士的寧),柱溫為30℃,進(jìn)樣量為10μl。結(jié)果:鹽酸偽麻黃堿、鹽酸麻黃堿、馬錢子堿和士的寧檢測(cè)質(zhì)量濃度線性范圍分別為0.916~18.320 0μg/ml(r=0.999 6)、10.034~200.680 0μg/ml(r=0.999 7)、8.693~121.700 0μg/ml(r=0.999 9)、9.696~193.920 0μg/ml(r=0.999 9);定量限分別為0.03、0.07、0.04、0.03 ng,檢測(cè)限分別為0.01、0.02、0.01、0.01 ng;精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD2.0%;加樣回收率分別為97.30%~99.79%(RSD=0.96%,n=6)、95.81%~99.36%(RSD=0.38%,n=6)、95.34%~99.93%(RSD=2.00%,n=6)、95.59%~99.80%(RSD=1.76%,n=6)。結(jié)論:該方法操作簡(jiǎn)便、結(jié)果準(zhǔn)確,可用于同時(shí)測(cè)定風(fēng)濕關(guān)節(jié)炎片中鹽酸麻黃堿、鹽酸偽麻黃堿、馬錢子堿、士的寧的含量。
[Abstract]:Objective: to establish a method for simultaneous determination of ephedrine hydrochloride, pseudoephedrine hydrochloride, brucine and strychnine in rheumatoid arthritis tablets. Methods: high performance liquid chromatography was used. The chromatographic column was XTerra-C_ (18). The mobile phase consisted of acetonitrile-0.01 mol/L sodium heptane sulfonate and 0.01 mol/L potassium dihydrogen phosphate (adjusted by phosphoric acid to 2.8) (gradient elution). The flow rate was 1. 0 ml/min,. The detection wavelength was 206 nm (ephedrine hydrochloride and pseudoephedrine hydrochloride), 260 nm (brucine and strychnine), the column temperature was 30 鈩，

本文編號(hào)：2325832